TAILIEUCHUNG - Isolation, characterization and identification of thiram degrading microorganisms from soil enrichment cultures

Mixed microbial cultures were obtained by enrichment from soil samples collected from the Gediz basin. A number of species of bacteria and fungi were isolated and partially characterized from enrichment clutures containing the fungicide thiram. | Turk J Biol 24 (2000) 353–363 © TÜBİTAK Isolation, Characterization and Identification of Thiram-degrading Microorganisms from Soil Enrichment Cultures Nurettin ŞAHİN Muğla University, Faculty of Arts and Sciences, Department of Biology, 48187, Kötekli, Muğla-TURKEY A. Üsame TAMER Celal Bayar University, Faculty of Arts and Sciences, Department of Biology, 45040, Manisa-TURKEY Received: Abstract: Mixed microbial cultures were obtained by enrichment from soil samples collected from the Gediz basin. A number of species of bacteria and fungi were isolated and partially characterized from enrichment clutures containing the fungicide thiram. According to their responses in morphological and biochemical tests fungi were identified as Aspergillus niger, A. flavus and Penicilium steckii. Bacterial isolates were assigned to the genera: Bacillus, Arthrobacter, Moraxella-like Acinetobacter and Streptomyces. Islates grew in a mineral salts medium containing mg/ml thiram and no growth factors were required. Key Words: Pesticide, fungicide, thiram (TMTD), biodegradation Zenginleştirilmiş Toprak Kültüründen Thiramı Parçalayan Mikroorganizmaların İzolasyonu, Karakterizasyonu ve Tanımlanması Özet: Karışık mikrobiyal kültürler Gediz havzasından alınan toprak örneklerinden zenginleştirme ile elde edildi. Fungusid thiram içeren zenginleştirilmiş kültürlerden fungus ve bakteri türleri izole edildi ve kısmen tanımlandı. Morfolojik ve biyokimyasal testlere verdikleri cevaplara göre funguslar; Aspergillus niger, A. flavus ve Penicillium steckii, olarak tanımlandı. Bakteriyal izolatlar; Bacillus, Arthrobacter, Moraxella-like Acinetobacter ve Streptomyces genuslarına dahil edildi. İzolatlar mg/ml thiram içeren mineral besiyerinde geliştiler ve büyüme faktörlerine ihtiyaç duymazlar. Anahtar Sözcükler: Pestisid, fungusid, thiram (TMTD), biyolojik parçalanma. Introduction Thiram [tetramethylthiuram disulfide (TMTD)] is one of the most widely applied dithiocarbamate .

• Sinh học
• Mixed microbial cultures
• Morphological and biochemical tests fungi
• Botrytis spp
• Soil enrichment cultures
• Bacillus mycoides
• Air lift reactor
• Column reactor
• Citric acid
• Citric acid production
• Immobilized polyextremophilic α Amylase
• Hypericum perforatum
• Antibacterial activity
• Antifungal activity
• Gram positive bacteria