TAILIEUCHUNG - In situ germination of pollen tetrads in Periploca graceae L. (Periplocaceae)

The mature anthers in large flower buds of Periploca graceae L. (Periplocaceae ) were examined cytologically. It was seen that pollen was retained in permanent tetrads. Pollen tetrads were usually observed as isobilateral, rarely as linear. It was also observed that pollen tetrads germinated within the anther loculi. | Turk J Biol 24 (2000) 337–343 © TÜBİTAK In Situ Germination of Pollen Tetrads in Periploca graceae L. (Periplocaceae) Feruzan DANE Trakya University, Faculty of Science and Letters, Department of Biology, Edirne-TURKEY Received: Abstract: The mature anthers in large flower buds of Periploca graceae L. (Periplocaceae ) were examined cytologically. It was seen that pollen was retained in permanent tetrads. Pollen tetrads were usually observed as isobilateral, rarely as linear. It was also observed that pollen tetrads germinated within the anther loculi. Most of the pollen tetrads were well stained and the germination rate was 90 %. In in situ germinated pollen tetrads, 1-4 pollen tubes were observed. During germination of pollen tetrads, the behavior of the nuclei was normal. At the early stage of pollen tube formation, a vegetative nucleus and two sperm cells were seen within the Pollen tubes. Pollen tubes were usually normal but rarely some abnormalities such as wall thickening, tube tip branching and extreme callose increase were also observed. Key Words:Periploca graceae, Pollen tetrad, In situ germination. Periploca graceae L. (Periplocaceae )’de Polen Tetradlarının In Situ Çimlenmesi Özet: Periploca graceae L. (Periplocaceae) bitkisinin büyük çiçek tomurcuklarındaki olgun anterler sitolojik olarak incelendi. Polenlerin tetradlar şeklinde bulunduğu görüldü. Polen tertradlarının genellikle isobilateral nadiren linear şeklinde olduğu gözlendi. Ayrıca polen tetradlarının anter lokusunda çimlendikleri görüldü. Tetrad polenlerinin iyi boyandığı ve çimlenme yüzdesinin 90 % gibi olduğu saptandı. In situ çimlenen polen tetradlarında oluşan tüp sayısının 1-4 arasında değiştiği gözlendi. Polen tetradlarının çimlenmesi sırasında nukleusların normal davranış gösterdikleri saptandı. Polen tüpü oluşumunun erken fazında tüpte vegetatif nukleus ve 2 sperm hücresi görüldü. Polen tüplerinde normal gelişimler gözlendiği gibi bazı anormallikler de gözlendi. Bu .

• Sinh học
• Periploca graceae
• Pollen tetrad
• In situ germination
• Tetrads germinated
• Examined cytologically