TAILIEUCHUNG - Susceptibility of chickpea (Cicer arietinum L.) cultivars to Agrobacterium tumefaciens (smith and townsend) conn
Twelve chickpea cultivars grown in Turkey were assessed for susceptibility to wild-type Agrobacterium tumefaciens strains A281 and A136NC. Although all chickpea cultivars showed susceptibility to infection from both A. tumefaciens strains, Diyar-95, Flip 87-8, İspanyol, Gökçe, Damla-89, and Aziziye-94 had higher frequencies of tumor formation from both strains than the other cultivars tested. | Turk J Biol 24 (2000) 285–289 © TÜBİTAK Susceptibility of Chickpea (Cicer arietinum L.) Cultivars to Agrobacterium tumefaciens (Smith and Townsend) Conn Aziz KARAKAYA Ankara Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü, Dışkapı, 06110, Ankara-TURKEY Sebahattin ÖZCAN Ankara Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü, Dışkapı, 06110, Ankara-TURKEY Received: Abstract: Twelve chickpea cultivars grown in Turkey were assessed for susceptibility to wild-type Agrobacterium tumefaciens strains A281 and A136NC. Although all chickpea cultivars showed susceptibility to infection from both A. tumefaciens strains, Diyar-95, Flip 87-8, İspanyol, Gökçe, Damla-89, and Aziziye-94 had higher frequencies of tumor formation from both strains than the other cultivars tested. Key Words: Agrobacterium tumefaciens, tumor formation, chickpea Nohut (Cicer arietinum L.) çeşitlerinin Agrobacterium tumefaciens (Smith and Townsend) Conn’e Hassasiyetleri Özet: Türkiye’de yetiştirilen 12 nohut çeşidinin doğal tip Agrobacterium tumefaciens streynleri A281 ve A136NC’ye karşı hassasiyetleri belirlenmiştir. Her iki streyn de kullanılan çeşitlerde ur oluşturmuş olup, çeşitler arasında farklılıklar gözlenmiştir. Çeşitlerin hepsinin A. tumefaciens enfeksiyonlarına hassasiyet göstermelerine karşın Diyar-95, Flip 87-8, İspanyol, Gökçe, Damla-89 ve Aziziye-94 çeşitleri test edilen diğer çeşitlere oranla her iki bakteri streyninde de yüksek oranlarda ur oluşturmuşlardır. Introduction Genetic engineering techniques provide valuable tools for improving crop plants (1). Gene transfer to higher plants via engineered Agrobacterium tumefaciens strains is now a routine technology (2). Agrobacterium tumefaciens is the causative agent of crown gall disease in numerous crops (3). Tumor formation is mediated by the Ti plasmid found in the bacterial cell (4, 5). Two plasmid and one chromosomal components are required for plant cell transformation (6). A small portion of this plasmid, .
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