TAILIEUCHUNG - Optimizing dna extraction condition from wood using response surface methods

The results showed the centrifugation time of hour and the volume ratio of isopropanol to solutions containing DNA of 1: were found to be the optimum conditions for the maximum DNA concentration of . The DNA extraction protocol with the optimum conditions was succeeded for dried wood samples. | Vietnam Journal of Science and Technology 55 (6) (2017) 725-733 DOI: OPTIMIZING DNA EXTRACTION CONDITION FROM WOOD USING RESPONSE SURFACE METHODS Du Phuc Thinh1, Tang Thi Kim Hong2, Huynh Van Biet3, * 1 2 Department of Biotechnology, Nong Lam University Ho Chi Minh City, 6 Quarter, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Viet Nam Center for Forestry Research and Technology Transfer, Nong Lam University Ho Chi Minh City, 6 Quarter, Linh Trung Ward, Thu Duc District, Ho Chi Minh city, Viet Nam 3 Research Institute for Biotechnology and Environment, Nong Lam University Ho Chi Minh City, 6 Quarter, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Viet Nam * Email: hvbiet@ Received: 25 April 2017; Accepted for publication: 31 October 2017 Abstract. The DNA extraction protocol from fresh wood, dried wood and leaves of Hopeaodorata by by CTAB method was investigated. The protocol optimization was performed through Central Composite Design (CCD) using Response Surface Methodology (RSM). The results showed the centrifugation time of hour and the volume ratio of isopropanol to solutions containing DNA of 1: were found to be the optimum conditions for the maximum DNA concentration of . The DNA extraction protocol with the optimum conditions was succeeded for dried wood samples. Keywords: DNA extraction, response surface methods, Hopeaodorata, psbA – trnH. 1. INTRODUCTION The DNA extraction is one of the first steps and plays an important role in study on genome of any species on earth. Depending on the purpose and object of study, DNA can be extracted from various tissues. For plants, DNA is usually extracted from the leaves, seeds and young buds, in which these tissues are the best DNA source and can be extracted easily. However, the collection of sample from mature trees, which are generally tall, is difficult and need more facilities. Consequently, it leads to be limited for the study .

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