TAILIEUCHUNG - Comparative analysis of DNA extracted from fish species preserved in formalin in two different periods

DNA was extracted from 2 cyprinid fishes kept under formalin preservation in 2 different time durations and the quality and quantity were analyzed. The tissue samples from specimens preserved for shorter duration yielded higher quantity of DNA compared to long-term preserved tissues. | M. RAJA, M. MURALIDHARAN, M. ARUNACHALAM Turk J Biol 35 (2011) 331-336 © TÜBİTAK doi: Comparative analysis of DNA extracted from fish species preserved in formalin in two different periods Manickam RAJA, Murugan MURALIDHARAN, Muthukumarasamy ARUNACHALAM Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi – 627 412, Tamil Nadu - INDIA Received: Abstract: DNA was extracted from 2 cyprinid fishes kept under formalin preservation in 2 different time durations, and the quality and quantity were analyzed. The tissue samples from specimens preserved for shorter duration yielded higher quantity of DNA compared to long-term preserved tissues. ANOVA (one way) on the quantity of the DNA from the samples of 2 periods showed that the genetic material obtained from the recently preserved samples was highly significant (P > ). The quality of the genetic material irrespective of the period of preservation was found to be the same. The purpose of the study was to check the viability of extracting genomic DNA from formalin preserved fish specimens and to estimate the quality and quantity. Key words: Cyprinid fishes, formalin preserved tissue, DNA extraction, quality analysis Introduction Species and population genetic assessment requires a reliable source of biological material (1). Preserved type specimens labeled with accurate identity and locality in museums and national repositories serve as authentic materials for taxonomic studies. These samples have been used for taxonomic studies using conventional methods of morphometric measurements and meristic counts. However, recent advancements in molecular biology with approaches like DNA sequencing have opened avenues ranging from evolutionary biology to forensic science. Taxonomists and systematists can use genome analysis to work out the relationship within species and branching patterns, hence a molecular approach targeting the genes encoded .

• Sinh học
• Cyprinid fishes
• Formalin preserved tissue
• DNA extraction
• Mitochondrial DNA
• Quality analysis
• Báo cáo sinh học hay
• cách trình bày báo cáo
• báo cáo sinh học
• công trình nghiên cứu sinh học
• tài liệu về sinh học
• BMC Genetics
• Biological characteristics
• Diploid hybrid fish
• Distant hybridization
• Ovary development
• Path coefficient
• Quality protein maize
• Grain yield
• Quality traits in quality protein maize
• Path coefficient analysis
• Correlation for yield contributing
• Quality traits
• Protein maize (Zea mays L )
• Path analysis studies
• Path analysis studies for yield
• Patients’ satisfaction of health service quality in public hospitals
• A PubHosQual analysis
• Health service quality in public hospitals
• The quality of health service
• Public hospitals in Jordan
• Quality parameters
• Path analysis
• Quality parameters in rice genotypes
• Rice genotypes
• Combining ability analysis
• Combining ability
• Combining ability analysis for earliness
• Yield and quality components
• Quality components in tomato
• Solanum lycopersicum L
• Abiotic factors
• Avifaunal diversity and water quality analysis
• Water quality analysis
• Inland pond
• Physico chemical factors are discussed
• Chicken breast
• Vacuum packaging
• 24 h storage
• Chilled chicken breast
• Quality analysis of vacuum packed
• Foxtail millet genotypes
• Setaria italica L
• Quality components
• Path analysis for grain yield
• Correlation and path analysis
• BMC Bioinformatics
• Quality trimming
• Paired end reads
• Diversity analysis
• Microbiome data analysis
• Soil analysis
• Water analysis
• Narmada valley
• Wildlife health by soil
• Tạp chí khoa học
• Service quality
• Technical and functional quality model
• Factor analysis
• Jetstar Pacific Airlines
• Genetic analysis
• Quality traits in bread wheat
• Bread wheat
• General combining ability
• Kolhapur city
• Microbial quality
• Microbial quality analysis
• Shrikhand sold in Kolhapur city
• Preparation of products
• Capsicum annuum L
• Graphical analysis
• Quality traits in sweet pepper
• Forty five F1 crosses generated
• Customer perceptions of service quality
• Life insurance companies
• Gap analysis and Multi regression
• Good quality of service
• Insurance company staff
• Line x tester
• Yield and quality
• Tester analysis in chilli
• Identification of superior parents
• Hybrids for yield
• Quality attributing traits
• Cell level quality control
• High throughput image analysis
• Image quality measurement
• Machine learning
• NPKS content in soil and curd
• Nutrient analysis
• NPKS content in soil
• Quality attributes of cauliflower
• NPKs on quality attributes
• Hybrid vigour
• Quality traits and yield
• Tester analysis carried over environments
• Estimation of hybrid vigour
• Quality traits of okra
• Ensuring product safety
• Lmproving quality in the supply chain
• Ebook Safety and quality issues
• Rapid detection of seafood toxins
• Understanding lipid oxidation in fish
• Underlying constructs affecting the quality of accounting human resources
• The quality of accounting human resources
• Quality of accounting human resources
• Human resource assessment
• Accounting service firms
• Genetic variability
• Genetic variability and D2 analysis
• D2 analysis for yield
• Quality traits in tomato
• Tomato (Solanum lycopersicum L )
• L x T analysis and chickpea
• Genetics analysis of yield
• Quality characters in chickpea
• Chickpea (Cicer arietinum L )
• Rice based cropping system
• American cotton
• Direct and indirect effect
• Fibre quality traits in Cotton
• Path coefficient analysis studies for yield
• Report on scientific research
• Automated Services Banks
• Quality ATM Banhks
• Quality Internet banking
• Services Telephone banking
• Confirmatory Factor Analysis