TAILIEUCHUNG - Determination and pathogenicity of the bacterial flora associated with the spruce bark beetle, Ips typographus L. (Coleoptera: Curculionidae: Scolytinae)

The Eurasian spruce bark beetle, Ips typographus L. (Coleoptera: Curculionidae: Scolytinae), is one of the most serious pests of spruce trees. We identified 8 bacterial isolates from this pest using conventional bacteriological tests (API 20E and API 50CH strips, and VITEK system (bioMerieux) analysis) and 16S rRNA gene sequence analysis. | Turk J Biol 35 (2011) 9-20 © TÜBİTAK doi: Determination and pathogenicity of the bacterial flora associated with the spruce bark beetle, Ips typographus (L.) (Coleoptera: Curculionidae: Scolytinae) Hacer MURATOĞLU, Kazım SEZEN, Zihni DEMİRBAĞ Department of Biology, Faculty of Arts and Sciences, Karadeniz Technical University, 61080 Trabzon - TURKEY Received: Abstract: The Eurasian spruce bark beetle, Ips typographus (L.) (Coleoptera: Curculionidae: Scolytinae), is one of the most serious pests of spruce trees. We identified 8 bacterial isolates from this pest using conventional bacteriological tests (API 20E and API 50CH strips, and VITEK system (bioMerieux) analysis) and 16S rRNA gene sequence analysis. Based on these studies, all isolates could be identified to the genus or species level as Bacillus sphaericus (It1), Acinetobacter sp. (It2), Kluyvera cryocrescens (It3), Acinetobacter sp. (It4), Vagococcus sp. (It5), Acinetobacter sp. (It6), Proteus vulgaris (It7), and Serratia liquefaciens (It8). We also evaluated the pathogenicity of these bacteria on adults of I. typographus. The 9 insecticidal activity of the bacterial isolates at a concentration of × 10 bacteria/mL, within 10 days, was for B. sphaericus (It1), for Acinetobacter sp. (It2 and It4), for P. vulgaris (It7), and for S. liquefaciens (It8). Since only It8 produced significantly increased mortality relative to the control, the bacterium S. liquefaciens may have potential as a biological control agent against the Eurasian spruce bark beetle. Key words: Bacterial identification, insecticidal activity, Ips typographus, microbial control Sekiz dişli kabuk böceği Ips typographus (L.) (Coleoptera: Curculionidae: Scolytinae)’un bakteriyal florasının belirlenmesi ve patojenitesi Özet: Sekiz dişli kabuk böceği, Ips typographus (L.) (Coleoptera: Curculionidae: Scolytinae), en önemli ladin zararlılarından biridir. Bu zararlıdan konvensiyonel .

• Sinh học
• Bacterial identification
• Insecticidal activity
• Ips typographus
• Microbial control
• VITEK system
• Sub clinical mastitis
• Antibiogram of bacterial isolates
• Bacterial isolates
• Bubaline subclinical mastitis
• Identification of bacterial pathogens
• Antibiotic sensitivity
• Exotic carps
• Indian major carps
• Molecular identification
• Bacterial species
• Indian major carp
• Exotic carps from south 24 Parganas
• River yamuna
• Water pollution
• Salmonella spp
• Vibrio spp
• Identification of bacterial strains
• Endophytic bacteria
• Plant growth promotion
• Identification of putative bacterial endophytes
• Plants in hot springs
• Vitrofor morphological features
• Staining Techniques
• Bacterial Cultivation
• Medical Microbiology
• Bacterial Genetics
• Viruses
• BMC Pediatrics
• Bacterial meningitis
• Bacterial pathogens identification
• Multiplex real time fluorescence quantitative
• Multiplex PCR based reverse line blot hybridization
• Accession number
• Klebsiella oxytoca
• Toxic effect on plants
• Reactive Red 11 decolorising
• Oxytoca CMGS 3 bacterial strain
• Biochemical identification
• Direct seeded rice
• Phyllosphere and methylobacterium
• Direct seeded rice growing areas
• PPFM bacterial isolates isolated
• Isolation and identification
• Cassava bacterial blight
• Xanthomonas axonopodis pv manihotis
• Cassava bacterial blight causing
• RpoD and gyrB genes
• Important crops in Vietnam for providing food
• Characterization of methicillin resistant bacterial isolates
• Methicillin resistant bacterial isolates
• Shellfish collected
• Cochin backwaters
• Aquatic organism
• Bovine calf
• Respiratory affections
• Himachal Pradesh
• Bacterial isolates from bovine calves
• BMC Bioinformatics
• Sequence analysis
• Bacterial genomics
• Gene presence absence
• Metabolic capability
• Salt tolerant bacteria
• Sodic soil
• Salt tolerant bacterial isolates
• Increase nutrient uptake
• Salt toerant bacteria
• BMC Genetics
• Bacterial fruit blotch
• Candidate gene
• Biotechnological approaches
• Plant diseases
• Chlorpyrifos degrading bacteria
• Rice upland crop soil
• The mekong delta
• The bacterial strains
• Swollen head syndrome
• Bacterial culture
• Chicken flock
• Identification of bacteria
• Microbial identification
• Marker genes
• Whole genome sequencing
• Bacterial isolate
• Biomerieux VITEK 2 system
• Staphylococcus sciuri
• Achromobacter xylosoxidans
• Vitek 2 compact system
• Bacterial leaf blight
• Disease severity
• SSR markers
• Wild rice
• Xanthomonas oryzae pv
• oryzae
• Reverse dot blot
• Bacterial intestinal pathogens
• PCR Reverse Dot Blot procedure
• Including Bacillus cereus
• Clostridium botulinum
• Clostridium perfringen
• BMC Veterinary Research
• Fish disease
• Mass spectrometry
• Infectious diseases
• Matrix assisted laser desorption
• Fish bacterial pathogens
• báo cáo hóa học
• công trình nghiên cứu về hóa học
• tài liệu về hóa học
• cách trình bày báo cáo
• báo cáo khoa học