TAILIEUCHUNG - Karyotype traits in Romanian selections of edible blue honeysuckle

Large morpho-anatomical and biochemical variability was evidenced in all Lonicera caerulea L. provenances. The knowledge of chromosome constitution is of basic importance in this context of elevated phenotype heterogeneity and represents a necessary step in breeding programs. | Turkish Journal of Biology Research Article Turk J Biol (2013) 37: 60-68 © TÜBİTAK doi: Karyotype traits in Romanian selections of edible blue honeysuckle 1, 1 1 2 Elena TRUTA *, Gabriela VOCHITA , Craita Maria ROSU , Maria Magdalena ZAMFIRACHE , 2 2 Zenovia OLTEANU , Lacramioara OPRICA 1 Department of Applied and Experimental Biology, NIRDBS-Institute of Biological Research, Iasi, Romania 2 Faculty of Biology, Alexandru Ioan Cuza University, Iasi, Romania Received: Accepted: Published Online: Printed: Abstract: Large morpho-anatomical and biochemical variability was evidenced in all Lonicera caerulea L. provenances. The knowledge of chromosome constitution is of basic importance in this context of elevated phenotype heterogeneity and represents a necessary step in breeding programs. The Romanian selections are tetraploid (2n = 4x = 36). Chromosomes are small-sized ( 2 increases) and from type A to type C (in relation to the ratio between the size of the largest and the smallest chromosome pair). . Idiogram construction An idiogram was drawn based on the average of the mean values calculated for the karyotypes of the 4 analyzed selections (Table 3). Short arms of the chromosomes were placed above the imaginary line representing centromere position. For each parameter, the mean and the standard error of the mean (x– ± SE) were calculated. 3. Results The small size of the chromosomes and the low occurrence of well-spread chromosomes in metaphase plates often TRUTA et al. / Turk J Biol – ± SE) of cytogenetic parameters of Romanian blue honeysuckle selections utilized for idiogram construction Table 3. Average values (x (CL = chromosome length, L = long arm length, S = short arm length, r = arm ratio (L/S), CI = centromeric index, RL = relative length). Pair Type CL L S r CI% RL% I sm ± ± ± ±

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• Blue honeysuckle
• Lonicera caerulea
• Asymmetry indexes
• Phenotype heterogeneity
• Restriction fragment length polymorphism
• Random amplified polymorphic DNA
• Genetic diversity
• DPPH (diphenyl picrylhydrazyl)
• ISSR markers
• Phenolic acids
• Random amplified polymorphic DNA
• Identification of genetic polymorphism
• Polymerase chain reaction
• Molecular biology techniques
• Sequence characterised amplified regions
• DNA Isolation
• RAPD primers
• Heat tolerance chickpea
• Molecular characterization
• Cicer arietinum L
• Sex discrimination
• Nile tilapia fish
• Oreochromis niloticus
• Genetical sex
• Molecular marker
• Random amplified polymorphic DNA fingerprinting
• Pleurotus strains
• Genetic diversity characterization
• Sodium nitrite
• Assessment of genetic polymorphism
• Genetic polymorphism
• Sugarcane somaclones
• Genomic template stability
• Tolerance index
• Hordeum vulgare L
• Genetic relationship
• HPV 16
• HPV 18
• Cervical cancer patient
• Human papilloma virus isolated
• Turkish Journal of Botany
• Tạp chí Thực vật học
• Long terminal repeat retrotransposons
• DNA methylation status
• Coupled restriction enzyme digestion random amplification
• Cephalaria Schrad
• Cephalaria sumbuliana
• Cephalaria davisiana
• Related taxa
• Amplified fragment length polymorphisms
• Molecular marker systems
• Sequence related amplified polymorphism
• Unweighted pair group method with arithmetic averages
• Sugar beet
• Molecular markers
• Polymorphic primers
• DNA markers
• RAPD markers
• Genetic linkage mapping
• Poncirus trifoliata L
• C
• reticulata Blanco
• tài liệu báo cáo nghiên cứu khoa học
• cách trình bày báo cáo
• kiến thức bệnh thú y
• báo cáo bệnh thú y
• các nghiên về bệnh thú ý
• Đề tài nghiên cứu tế bào gốc
• Tiểu luận công nghệ sinh học
• Đề tài kỹ thuật Rapd
• Báo cáo thực hành hóa sinh
• Luận văn nghiên cứu đa dạng sinh học
• Tiểu luận Kỹ thuật di truyền
• Cotton Gossypium hirsutum L
• Polymerase chain reaction method
• Gossypium genotypes
• Genetic relationships
• Velezia L
• Unweighted pair group method
• RAPD technique
• Plant genetic resources
• RAPD dendrogram
• Phenotypic similarity
• Terrestrial orchids
• Principal component analysis
• Principal coordinate analysis
• Natural source
• Primer combinations
• Green gram genotypes
• Morphological markers
• Simple sequence repeat
• Tạp chí khoa học
• Mối quan hệ di truyền
• Kỹ thuật RAPD
• Quan hệ di truyền
• Đa dạng di truyền
• Giống đậu tương
• DNA đa hình
• Random Amplified Polymorphic
• Nhân bản ngẫu nhiên
• Hệ số tương đồng di truyền
• Chickpea Cicer arietinum L
• Root rot
• Pathogen free seed
• Genetic diversity analysis of citrus cultivars
• Bellis perennis
• RAPD PCR analysis
• Genomic structures of species
• Rhaponticoides iconiensis
• Oxytona Bernh
• Papaver accessions
• Oxytona species
• Polymerase chain reactions