TAILIEUCHUNG - Lecture Molecular biology (Fifth Edition): Chapter 17 - Robert F. Weaver

This chapter concerns the initiation of translation in eukaryotes and bacteria. Because the nomenclatures of the two systems are different, it is easier to consider them separately. Therefore, let us begin with a discussion of the simpler system, initiation in bacteria. Then we will move on to the more complex eukaryotic scheme. | Molecular Biology Fifth Edition Chapter 17 The Mechanism of Translation I: Initiation Lecture PowerPoint to accompany Robert F. Weaver Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Translation Translation is the process by which ribosomes read the genetic message in mRNA and produce a protein product according to the message Ribosomes are protein factories Transfer RNAs (tRNAs) play an important role as adaptors that can bind and amino acid at one end and interact with the mRNA at the other end 17- Initiation of Translation in Bacteria Two important events must occur before translation initiation can take place Generate a supply of aminoacyl-tRNAs Amino acids must be covalently bound to tRNAs Process of bonding tRNA to amino acid is called tRNA charging Dissociation of ribosomes into their two subunits The cell assembles the initiation complex on the small ribosomal subunit The two subunits must separate to make assembly possible

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• tRNA charging
• Initiation in Eukaryotes
• High Yield cell and molecular biology
• Cell and molecular biology
• The cell cycle
• Molecular biology of cancer
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• Molecular biology techniques
• Identification of human disease genes
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• Chromosome replication
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• The human nuclear genome
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• Molecular cloning methods
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• Amino acids
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• Studying genes
• History of genetics
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• Mendel’s Laws
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• Mechanism of transcription
• RNA polymerase structure
• Lac operon
• Bacterial transcription
• Sigma factor switching
• DNA Protein interactions
• The λ family of repressors
• Eukaryotic RNA polymerases
• RNA polymerases
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• Transcription activators in eukaryotes
• RNA polymerase II
• Histones in the nucleosome
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• Messenger RNA processing I
• Genes in pieces
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