TAILIEUCHUNG - Báo cáo khoa học: Identification of sites of phosphorylation by G-protein-coupled receptor kinase 2 in b-tubulin

G-protein-coupled receptor kinase 2 (GRK2) is known to specifically phosphorylate the agonist-bound forms of G-protein-coupled receptors (GPCRs). This strict specificity is due at least partly to activation of GRK2 by agonist-bound GPCRs, in which basic residues in intracellular regions adjacent to transmembrane segments are thought to be involved. Tubulin was found to be phosphorylated by GRK2, but it remains unknown if tubulin can also serve as both a substrate and an activator for GRK2. | Eur. J. Biochem. 270 1154-1163 2003 FEBS 2003 doi Identification of sites of phosphorylation by G-protein-coupled receptor kinase 2 in b-tubulin Norihiro Yoshida1 I Kazuko Haga1 2 and Tatsuya Haga1 2 1 Department of Neurochemistry Faculty of Medicine University of Tokyo Japan 2Institute for Biomolecular Science Faculty of Science Gakushuin University Tokyo Japan G-protein-coupled receptor kinase 2 GRK2 is known to specifically phosphorylate the agonist-bound forms of G-protein-coupled receptors GPCRs . This strict specificity is due at least partly to activation of GRK2 by agonistbound GPCRs in which basic residues in intracellular regions adjacent to transmembrane segments are thought to be involved. Tubulin was found to be phosphorylated by GRK2 but it remains unknown if tubulin can also serve as both a substrate and an activator for GRK2. Purified tubulin phosphorylated by GRK2 was subjected to biochemical analysis and the phosphorylation sites in b-tubulin were determined to be Thr409 and Ser420. In addition the Ser444 in bm-tubulin was also indicated to be phosphorylated by GRK2. The phosphorylation sites in tubulin for GRK2 reside in the C-terminal domain of b-tubulin which is on the outer surface of microtubules. Pretreatment of tubulin with protein phosphatase type-2A PP2A resulted in a twofold increase in the phosphorylation of tubulin by GRK2. These results suggest that tubulin is phosphorylated in situ probably by GRK2 and that the phosphorylation may affect the interaction of microtubules with microtubule-associated proteins. A GST fusion protein of a C-terminal region of bI-tubulin 393-445 residues containing 19 acidic residues but only one basic residue was found to be a good substrate for GRK2 like full-length b-tubulin. These results together with the finding that GRK2 may phosphorylate synuclein and phosducin in their acidic domains indicate that some proteins with very acidic regions but without basic activation .

• Báo cáo khoa học
• scientific reports
• Report medicine
• traditional medicine
• breast cancer
• biological activities
• environmental medicine
• collection of scientific reports
• industry reports philosophy of history reports
• reports in literature
• a scientific b shirt
• presentation of scientific reports
• reports on literature
• philosophy statement
• documents
• studies English language and English language communication and English language commercial
• progress reports at scientific collections
• agronomic reports
• reports in physics
• biology reports
• thematic biological
• induced geometry
• mathematical topics
• quantum mechanics
• physics
• reporting mathematics
• physics reports
• livestock reports
• physical reports
• research physicist
• specializing in physics
• mathematical statements
• mathematical equations
• English pronunciation
• presentation report
• agricultural reports
• technical crops
• bio technology
• English reports
• scientific research
• presentation reports
• mathematical knowledge
• reports of mathematics
• represented
• Wilf on packing distinct
• Tur´n density
• mathematical works
• patterns in a permutation
• statements and documents scientific reports
• reports agronomy
• biotechnology
• plants Vietnam
• specialized biological
• microbiological methods
• presenting the report
• anti bacterial properties of shrimp
• energy exchange
• engineering chicken
• Path counting
• random matrix theory
• Even circuits
• prescribed clockwise parity
• Correspondence between
• algorithmic characterizations
• Finding Induced Acyclic
• Random Digraphs
• Nonexistence results
• Hadamard like matrices
• Voltage Graphs
• Group Presentations
• Cages mathematical knowledge
• Dominance Order
• Graphical Partitions
• On the functions
• values in [α(G
• Box With Translates
• Two Given Rectangular Brick
• On the q analogue
• the sum of cubes