TAILIEUCHUNG - Báo cáo sinh học: " Exploring internal features of 16S rRNA gene for identification of clinically relevant species of the genus Streptococcus"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí y học Molecular Biology cung cấp cho các bạn kiến thức về ngành sinh học đề tài: Exploring internal features of 16S rRNA gene for identification of clinically relevant species of the genus Streptococcus. | Lal et al. Annals of Clinical Microbiology and Antimicrobials 2011 10 28 http content 10 1 28 ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS RESEARCH Open Access Exploring internal features of 16S rRNA gene for identification of clinically relevant species of the genus Streptococcus Devi Lal Mansi Verma and Rup Lal Abstract Background Streptococcus is an economically important genus as a number of species belonging to this genus are human and animal pathogens. The genus has been divided into different groups based on 16S rRNA gene sequence similarity. The variability observed among the members of these groups is low and it is difficult to distinguish them. The present study was taken up to explore 16S rRNA gene sequence to develop methods that can be used for preliminary identification and can supplement the existing methods for identification of clinically-relevant isolates of the genus Streptococcus. Methods 16S rRNA gene sequences belonging to the isolates of S. dysgalactiae S. equi S. pyogenes S. agalactiae S. bovis S. gallolyticus S. mutans S. sobrinus S. mitis S. pneumoniae S. thermophilus and S. anginosus were analyzed with the purpose to define genetic variability within each species to generate a phylogenetic framework to identify species-specific signatures and in-silico restriction enzyme analysis. Results The framework based analysis was used to segregate Streptococcus spp. previously identified upto genus level. This segregation was validated using species-specific signatures and in-silico restriction enzyme analysis. 43 uncharacterized Streptococcus spp. could be identified using this approach. Conclusions The markers generated exploring 16S rRNA gene sequences provided useful tool that can be further used for identification of different species of the genus Streptococcus. Keywords phylogenetic framework signature sequences genetic heterogeneity Background The genus Streptococcus consists of spherical Gram positive bacteria

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