TAILIEUCHUNG - Handbook of Microbiological Media, Fourth Edition part 34

Handbook of Microbiological Media, Fourth Edition part 34 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Castenholz D Medium 325 Preparation of Medium Add components except blood and water-lysed blood solution to distilled deionized water and bring volume to . Mix thoroughly. Gently heat to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 C. Aseptically add of sterile blood and of sterile water-lysed blood solution one part blood to three parts water . Water-lysed blood may be omitted if sterile blood is partially lysed due to storage. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the isolation of fastidious bacteria from clinical specimens. For the cultivation under reduced oxygen tension of fastidious microorganisms such as Haemophilus influenzae Neisseria meningitidis and Neisseria gonorrhoeae. Casman HiVeg Broth Base with Blood Composition per liter Plant hydrolysate No. Plant peptone No. Plant Corn p-Amino benzoic acid PABA . Water-lysed blood pH at 25 C Source This medium without blood and water-lysed blood solution is available as a premixed powder from HiMedia. Water-Lysed Blood Solution Composition per Preparation of Water-Lysed Blood Solution Add blood to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Preparation of Medium Add components except blood and water-lysed blood solution to distilled deionized water and bring volume to . Mix thoroughly. Gently heat to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 C. Aseptically add of sterile blood and of sterile water-lysed blood solution one part blood to three parts water . Water-lysed blood may be omitted if sterile blood is partially lysed due to storage. Mix thoroughly. Use For the cultivation of fastidious bacteria from clinical specimens. For the cultivation under reduced oxygen tension of fastidious .

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