TAILIEUCHUNG - Handbook of Microbiological Media, Fourth Edition part 85

Handbook of Microbiological Media, Fourth Edition part 85 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | HiCrome ECC Agar 835 Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes. Use For the simultaneous detection of Escherichia coli in foods without further confirmation on membrane filter or by indole reagent. HiCrome E. coli Agar Composition per liter Casein enzymic Bile salts pH at 25 C Source This medium is available as a premixed powder from Hi-Media. Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes. Use For the simultaneous detection of Escherichia coli in foods without further confirmation on membrane filter or by indole reagent. HiCrome E. coli Agar A E. coli Agar A HiCrome Composition per liter Casein enzymic Peptone Bile salts mixture . X-Glucuronide . pH at 25 C Source This medium is available as a premixed powder from Hi-Media. Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 C. Pour into sterile Petri dishes. Use For the detection and enumeration of Escherichia coli in foods without further confirmation on membrane filter or by indole reagent. The chromogenic agent X-glucuronide used in this medium helps to detect glucuronidase activity. E. coli cells absorb X-glucuronide and the intracellular glucuronidase splits the bond between the chromophore and the glucuronide. The released chromophore gives coloration to the colonies. Bile

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