TAILIEUCHUNG - báo cáo khoa học: "Pig gene mapping : assignment of the genes for mannosephosphate isomerase (MPI) and nucleoside phosphorylase (NP) to chromosome no"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Pig gene mapping : assignment of the genes for mannosephosphate isomerase (MPI) and nucleoside phosphorylase (NP) to chromosome no | Génét. Sél. Evol. 1986 18 4 375-384 Pig gene mapping assignment of the genes for mannosephosphate isomerase MPI and nucleoside phosphorylase NP to chromosome no. 7 G. DOLF and G. STRANZINGER Federal Institute of Technology Institute of Animal Sciences CH-8092 Zurich Switzerland Summary Ten hybrid cell lines pig X a3 Chinese hamster have been investigated with respect to their content of pig chromosomes and the enzymes MPI and NP. The pig chromosomes have been identified by their Q-band pattern. The enzymes were analysed by cellulose acetate gel electrophoresis. The results indicate that the genes for MPI and NP are located on chromosome no. 7. Key words Pig gene mapping mannosephosphate isomerase MPI nucleoside phosphorylase HP . Resume Carte génique du pore assignation des genes de la mannosephosphate isomerase MPI et de la nucleoside phosphorylase NP au chromosome n 7 La presence de chromosomes porcins et des enzymes MPI et NP a été recherchée dans 10 lignées de cellules hybrides pore X a3 hamster chinois . Les chromosomes porcins ont été identiíiés grâce à leurs bandes Q caractéristiques et les enzymes analysés par électrophorèse sur gel d acétate de cellulose. Les résultats indiquent que les gènes codant pour MPI et NP sont situés sur le chromosome n 7. Mots clés Pore carte génique mannosephosphate isomérase MPI nucléoside phosphorylase NP . I. Introduction The present State of the pig gene map includes roughly 40 genes. Most of them belong to linkage groups whereas only a few genes have been assigned directly to a chromosome. The genes for HPRT G6PD PGK and GLA have been located on the Reprint request Prof. Dr. G. Stranzinger. 376 G. DOLF G. STRANZINGER X-chromosome by means of somatic cell hybrids Foerster et al. 1980 Gellin et al. 1980 Leong et al. 1983a . The diseases splayleg Lax 1971 and tremor AIII Harding et al. 1973 are also associated with the X-chromosome. Autosomal assignments were made by the methods of family analysis in situ hybridization and .

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