TAILIEUCHUNG - Báo cáo sinh học: " Biodistribution and blood clearance of plasmid DNA administered in arginine peptide complexes"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Biodistribution and blood clearance of plasmid DNA administered in arginine peptide complexes | Woo et al. Genetic Vaccines and Therapy 2011 9 13 http content 9 1 13 GENETIC VACCINES AND THERAPY RESEARCH Open Access Biodistribution and blood clearance of plasmid DNA administered in arginine peptide complexes Jung Gyu Woo Na Young Kim Jai Myung Yang and Sungho Shin Abstract Background Peptide DNA complexes have great potential as non-viral methods for gene delivery. Despite promising results for peptide-mediated gene delivery technology an effective systemic peptide-based gene delivery system has not yet been developed. Methods This study used pCMV-Luc as a model gene to investigate the biodistribution and the in vivo efficacy of arginine peptide-mediated gene delivery by polymerase chain reaction PCR . Results Plasmid DNA was detected in all organs tested 1 h after intraperitoneal administration of arginine DNA complexes indicating that the arginine DNA complexes disseminated widely through the body. The plasmid was primarily detected in the spleen kidney and diaphragm 24 h post administration. The mRNA expression of plasmid DNA was noted in the spleen kidney and diaphragm for up to 2 weeks and in the other major organs for at least 1 week. Blood clearance studies showed that injected DNA was found in the blood as long as 6 h after injection. Conclusions Taken together our results demonstrated that arginine DNA complexes are stable in blood and are effective for in vivo gene delivery. These findings suggest that intraperitoneal administration of arginine DNA complexes is a promising tool in gene therapy. Keywords Arginine peptide Biodistribution Gene therapy Peptide vector Systemic gene delivery Background Cell-penetrating peptides CPPs have been widely shown to transfer macromolecules into living cells 1 2 . Several of these peptides have been identified such as Tat 3 Antp 4 and VP22 5 . Carrier peptides which are fused to their cargo molecules provide a method for delivering intracellularly acting proteins or nucleic acids to cells in .

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