TAILIEUCHUNG - Báo cáo sinh học: "Recombinant λ-phage nanobioparticles for tumor therapy in mice models"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Recombinant λ-phage nanobioparticles for tumor therapy in mice models | Ghaemi et al. Genetic Vaccines and Therapy 2010 8 3 http content 8 1 3 GENETIC VACCINES AND THERAPY RESEARCH Open Access Recombinant Ă-phage nanobioparticles for tumor therapy in mice models Amir Ghaemi 1 6 Hoorieh Soleimanjahi 1 Pooria Gill2 Zuhair Hassan3 Soodeh Razeghi M Jahromi4 and Farzin Roohvand5 Abstract Lambda phages have considerable potential as gene delivery vehicles due to their genetic tractability low cost safety and physical characteristics in comparison to other nanocarriers and gene porters. Little is known concerning lambda phage-mediated gene transfer and expression in mammalian hosts. We therefore performed experiments to evaluate lambda-ZAP bacteriophage-mediated gene transfer and expression in vitro. For this purpose we constructed recombinant Ầ-phage nanobioparticles containing a mammalian expression cassette encoding enhanced green fluorescent protein EGFP and E7 gene of human papillomavirus type 16 Ầ-HPV-16 E7 using Lambda ZAP- CMV XR vector. Four cell lines COS-7 CHO TC-1 and HEK-239 were transduced with the nanobioparticles. We also characterized the therapeutic anti-tumor effects of the recombinant Ầ-HPV-16 E7 phage in C57BL 6 tumor mice model as a cancer vaccine. Obtained results showed that delivery and expression of these genes in fibroblastic cells COS-7 and CHO are more efficient than epithelial cells TC-1 and HEK-239 using these nanobioparticles. Despite the same phage entry the internalizing titers of COS-7 and CHO cells were more than TC-1 and HEK-293 cells respectively. Mice vaccinated with Ầ-HPV-16 E7 are able to generate potent therapeutic antitumor effects against challenge with E7-expressing tumor cell line TC-1 compared to group treated with the wild phage. The results demonstrated that the recombinant Ầ-phages due to their capabilities in transducing mammalian cells can also be considered in design and construction of novel and safe phage-based nanomedicines. Introduction Different strategies .

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