TAILIEUCHUNG - Báo cáo sinh học: "Respiratory syncytial virus infection in Fischer 344 rats is attenuated by short interfering RNA against the RSV-NS1 gene"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Respiratory syncytial virus infection in Fischer 344 rats is attenuated by short interfering RNA against the RSV-NS1 gene | Genetic Vaccines and Therapy BioMed Central Research Open Access Respiratory syncytial virus infection in Fischer 344 rats is attenuated by short interfering RNA against the RSV-NS1 gene Xiaoyuan Kong 1 Weidong Zhang1 Richard F Lockey2 Alexander Auais3 Giovanni Piedimonte4 and Shyam S Mohapatra1 Address University of South Florida College of Medicine Florida USA 2James A Haley VA Hospital Tampa Florida USA 3Batchelor Children s Institute University of Miami Miami Florida USA and 4West Virginia University Morgantown WV USA Email Xiaoyuan Kong - xkong@ Weidong Zhang - wzhang@ Richard F Lockey - rlockey@ Alexander Auais - aauais@ Giovanni Piedimonte - Shyam S Mohapatra - smohapat@ Corresponding author Published I February 2007 Received 27 July 2006 _ I- . Accepted 1 February 2007 Genetic Vaccines and Therapy 2007 5 4 doi 186 1479-0556-5-4 This article is available from http content 5 1 4 2007 Kong et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Respiratory syncytial virus RSV causes severe bronchiolitis and is a risk factor for asthma. Since there is no commercially available vaccine against RSV a short interfering RNA against the RSV-NS1gene siNS1 was developed and its potential for decreasing RSV infection and infection-associated inflammation in rats was tested. Methods Plasmids encoding siNS1 or an unrelated siRNA were complexed with a chitosan nanoparticle delivery agent and administered intranasally. Control animals received a plasmid for a non-specific siRNA. After expression of the plasmid in lung cells for 24 hours the rats were intranasally infected with RSV. Results .

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