TAILIEUCHUNG - Báo cáo sinh học: "Immunological analysis of a Lactococcus lactis-based DNA vaccine expressing HIV gp120"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Immunological analysis of a Lactococcus lactis-based DNA vaccine expressing HIV gp120 | Genetic Vaccines and Therapy BioMed Central Research Immunological analysis of a Lactococcus lactis-based DNA vaccine expressing HIV gp120 Gregers J Gram1 Anders Fomsgaard1 Mette Thorn1 Soren M Madsen2 and Jacob Glenting 2 Open Access Address Department of Virology State Serum Institute Artillerivej 5 DK-2300 Copenhagen Denmark and 2Vaccine Technology Bioneer A S Kogle Alle 2 DK-2970H0rsholm Denmark Email Gregers J Gram - gjg@ Anders Fomsgaard - afo@ Mette Thorn - mth@ Soren M Madsen - sma@ Jacob Glenting - jag@ Corresponding author Published 29 January 2007 Received 21 October 2006 J n I I r 11 m M- r r- Accepted 29 January 2007 Genetic Vaccines and Therapy 2007 5 3 doi 186 1479-0556-5-3 This article is available from http content 5 1 3 2007 Gram et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract For reasons of efficiency Escherichia coli is used today as the microbial factory for production of plasmid DNA vaccines. To avoid hazardous antibiotic resistance genes and endotoxins from plasmid systems used nowadays we have developed a system based on the food-grade Lactococcus lactis and a plasmid without antibiotic resistance genes. We compared the L. lactis system to a traditional one in E. coli using identical vaccine constructs encoding the gp 1 20 of HIV-1. Transfection studies showed comparable gp120 expression levels using both vector systems. Intramuscular immunization of mice with L. lactis vectors developed comparable gp120 antibody titers as mice receiving E. coli vectors. In contrast the induction of the cytolytic response was lower using the L. lactis vector. Inclusion of CpG motifs in the plasmids increased T-cell activation more when

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