TAILIEUCHUNG - PROSTATE CANCER – DIAGNOSTIC AND THERAPEUTIC ADVANCES

Monoclonal antibodies directed against PSA clone 28A4 (Novacastro, UK) in a concentration of 2,5μg/ml were used to detect prostate cells, and identified using a detection system based on alkaline phosphatase-antialkaline phosphatase (LSAB2 DAKO, USA) with new-fuschin as the chromogen. To permit the rapid identification of positive cells there was no counter staining with Mayer´s hematoxilin. Levisamole (DAKO, USA) was used as an inhibitor of endogenous alkaline phosphatase, with positive and negative controls. Positive samples underwent a second stage of processing, using the monoclonal antibody against P504S clone13H4 (Novocastro, UK) and a system of detection based on peroxidase (LSAB2, DAKO, USA) with Vector VIP (Vector, USA) as the chromogen. Endogenous. | PROSTATE CANCER -DIAGNOSTIC AND THERAPEUTIC ADVANCES Edited by Philippe E. Spiess Prostate Cancer - Diagnostic and Therapeutic Advances Edited by Philippe E. Spiess Published by InTech Janeza Trdine 9 51000 Rijeka Croatia Copyright 2011 InTech All chapters are Open Access articles distributed under the Creative Commons Non Commercial Share Alike Attribution license which permits to copy distribute transmit and adapt the work in any medium so long as the original work is properly cited. After this work has been published by InTech authors have the right to republish it in whole or part in any publication of which they are the author and to make other personal use of the work. Any republication referencing or personal use of the work must explicitly identify the original source. Statements and opinions expressed in the chapters are these of the individual contributors and not necessarily those of the editors or publisher. No responsibility is accepted for the accuracy of information contained in the published articles. The publisher assumes no responsibility for any damage or injury to persons or property arising out of the use of any materials instructions methods or ideas contained in the book. Publishing Process Manager Marija Radja Technical Editor Teodora Smiljanic Cover Designer Jan Hyrat Image Copyright Bocos Benedict 2011. Used under license from First published November 2011 Printed in Croatia A free online edition of this book is available at Additional hard copies can be obtained from orders@ Prostate Cancer - Diagnostic and Therapeutic Advances Edited by Philippe E. Spiess p. cm. ISBN 978-953-307-319-4 ikrrr iJ OPEN ACCESS INI Cvn PUBLISHER INTECH open free online editions of InTech Books and Journals can be found at .

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