TAILIEUCHUNG - Báo cáo sinh học: " Substitution of the α-lactalbumin transcription unit by a CAT cDNA within a BAC clone silenced the locus in transgenic mice without affecting the physically linked Cyclin T1 gene"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: Substitution of the α-lactalbumin transcription unit by a CAT cDNA within a BAC clone silenced the locus in transgenic mice without affecting the physically linked Cyclin T1 gene | 239 Genet Sei. Evol. 35 2003 239-247 INRA EDP Sciences 2003 DOI gse 2003006 Original article Substitution of the a-lactalbumin transcription unit by a CAT cDNA within a BAC clone silenced the locus in transgenic mice without affecting the physically linked Cyclin T1 gene Solange Souliera Marthe Hudrisiera José Costa Da Silvaa Caroline Maederb Celine Vigliettab Nathalie Besnarda Jean-Luc Vilottea a Laboratoire de génétique biochimique et de CYtogénétique Departement de génétique animale Institut national de la recherche agronomique 78352 JouY-en-Josas Cedex France b Laboratoire de biologie du développement et de biotechnologie Departement de phYsiologie animale Institut national de la recherche agronomique 78352 JouY-en-Josas Cedex France Received 12 December 2001 accepted 1 October 2002 Abstract - We recently reported that a goat bacterial artificial chromosome BAC clone conferred site-independent expression in transgenic mice of the two loci present within its insert the ubiquitously expressed Cyelin T1 and the mammary specific a-lactalbumin alac genes. To assess if this vector could target mammary-restricted expression of cDNA the CAT ORF was introduced by homologous recombination in Escherichia eoli in place of the alac transcription unit. The insert of this modified BAC was injected into mice and three transgenic lines were derived. None of these lines expressed the CAT gene suggesting that the use of long genomic inserts is not sufficient to support the expression of intron-less transgenes. The physically linked goat Cyelin T1 locus was found to be active in all three lines. This observation reinforced the hypothesis that the two loci are localised in two separate chromatin domains. bacterial artificial chromosome homologous recombination intron transgenic mice chromatin domain Correspondence and reprints E-mail vilotte@ 240 s. Soulier et al. 1. introduction The use of long genomic fragments such as bacterial artihcial chromosome BAC or .

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