TAILIEUCHUNG - Determination of the earliest time for heat shock inducing osteoporosis like phenotype in the rankl:HSE:CFP medaka fish model for osteoporosis

In this study, we investigated whether osteoporosis-like phenotype could be induced in the transgenic larvae when heat-shock was applied at earlier time points, namely when larvae were at 1,2,3,4,5, or 6 dpf. The obtained results show that heat-shocks from the time point of 3 dpf onwards resulted in osteoporosis-like phenotype, while heat-shock at 1 or 2 dpf did not affect mineralized bone matrix in 11 dpf larvae. | VNU Journal of Science Natural Sciences and Technology Vol. 37 No. 3 2021 37-43 Original Article Determination of the Earliest Time for Heat-shock Inducing Osteoporosis-like Phenotype in the rankl HSE CFP Medaka Fish Model for Osteoporosis To Thanh Thuy Phan Cong Son Tran Duc Long VNU University of Science 334 Nguyen Trai Thanh Xuan Hanoi Vietnam Received 10 August 2021 Revised 23 August 2021 Accepted 30 August 2021 Abstract The transgenic medaka rankl HSE CFP expressing Rankl a stimulator for osteoclastogenesis - the formation and activation of osteoclasts bone eating cells under the control of a heat inducible promotor has been established as a model for osteoporosis to evaluate antiosteoporosis effects of substances. Transgenic larvae are usually heat-shocked for 90 minutes at 39 oC when they are at 9 days post fertilization dpf and osteoporosis-like phenotype is analysed when larvae are at 11 dpf. In this study we investigated whether osteoporosis-like phenotype could be induced in the transgenic larvae when heat-shock was applied at earlier time points namely when larvae were at 1 2 3 4 5 or 6 dpf. The obtained results show that heat-shocks from the time point of 3 dpf onwards resulted in osteoporosis-like phenotype while heat-shock at 1 or 2 dpf did not affect mineralized bone matrix in 11 dpf larvae. These provide important evidence for study of onset of Rankl induced osteoclasts in fish and help improve experimental procedures using this fish model for osteoporosis. Keywords Medaka heat-shock osteoclasts rankl osteoporosis fish model. 1. Introduction people diagnosed with the disease and the number of affected people is increasing Osteoporosis is a common bone disorder dramatically due to aging population and lack characterized by decrease in bone density and of effective and safe drugs and therapies 2 . structure resulting in elevated risk of bone Animal models are always important for fracture. This disease is most prevalent in research to develop better

• Sinh học
• Natural sciences and Technology
• Fish model
• The earliest time
• Heat shock inducing osteoporosis like
• Medaka fish model
• Natural sciences
• Technology sciences
• Vecny este epoxy alkyd
• Tính chất điện
• Độ bền dung môi
• Độ bền nhiệt
• Detailed checklist
• Field labels
• Grammitid specimens
• VNU University of Science
• Xác định biên
• Dị thường từ
• Dị thường trọng lực
• Bắc Việt Nam
• Mỡ cá tra
• Etyl cacboxylate
• Etyl biodiesel
• Este axit omega 3 6 9
• Phương pháp đồng dung môi
• Đồng dung môi
• Hợp chất chứa dị vòng
• Chất xúc tác
• Phản ứng Sonogashira
• Hợp chất dị vòng năm cạnh
• Phản ứng Click
• Nano đồng
• Nano silica
• Khả năng kháng nấm
• Pyricularia oryzae
• Rhizoctonia solani
• Phân loại thực vật
• Lạng Sơn
• Aspidistra papillata
• Plant taxonomy
• Arachniodes simplicior
• Flora of Vietnam
• New record
• Phản ứng ngưng tụ croton
• Hoạt tính kháng khuẩn
• Chương trình PASS
• Dẫn xuất thiopodand
• Vật liệu nano
• Tính chất quang
• Tính chất từ
• Vật liệu nano HoFeO3
• Cảm biến miễn dịch điện hóa
• Kháng thể IgY
• Virus Newcastle
• Bình phản ứng mini
• Xúc tác lưỡng kim loại Co2Cu2
• CI NEB cơ chế phản ứng
• Phản ứng hydrogen hóa carbon monoxide
• Bọ rùa hai mảng đỏ
• Lemnia biplagiata
• Tỷ lệ sống sót
• Tỷ lệ vào nhộng
• Năng ăn con mồi
• Chất lượng nước
• Động vật nổi
• Thành phần loài
• Kênh Bún Xáng
• Đa dạng sinh học
• Camphor
• science and technology
• scientific reports
• research projects
• scientific research
• Immune cells expansion
• Peripheral blood
• Immune cell therapy
• Natural killer cells
• Cytotoxic T lymphocytes
• Peripheral blood mononuclear cells
• genus Arisaema Mart
• Technology
• Litsea glutinosa
• Chemical composition
• Vietnamese using RFLP PCR
• Assessment of OPRM1
• Dioxin contamination
• carbazol degrading bacteria
• Obtaining Essential Oil
• Shikimic Acid
• cyanobacterial blooms
• freshwater bodies
• blood insulin secretion
• Fortunella japonica
• Docynia indica