TAILIEUCHUNG - Deletion of high-molecular-weight glutenin subunits in wheat significantly reduced dough strength and bread-baking quality

High-molecular-weight glutenin subunits (HMW-GS) play important roles in the elasticity of dough made from wheat. The HMW-GS null line is useful for studying the contribution of HMW-GS to the end-use quality of wheat. | Nội dung Text Deletion of high-molecular-weight glutenin subunits in wheat significantly reduced dough strength and bread-baking quality .den color inherit .ttnd ol .ttnd ul .ttnd dl padding 0 0px 0 20px .ttnd hr margin 10px 0px .ttnd a href javascript void 0 .ttnd a href color inherit dtextscript p text-align left dtextscript img vertical-align middle Zhang et al. BMC Plant Biology 2018 18 319 lt br gt https s12870-018-1530-z lt br gt lt br gt lt br gt lt br gt lt br gt RESEARCH ARTICLE Open Access lt br gt lt br gt Deletion of high-molecular-weight glutenin lt br gt subunits in wheat significantly reduced lt br gt dough strength and bread-baking quality lt br gt Yingjun Zhang1 Mengyun Hu1 Qian Liu1 Lijing Sun1 Xiyong Chen1 Liangjie Lv1 Yuping Liu1 Xu Jia2 lt br gt and Hui Li1 lt br gt lt br gt lt br gt Abstract lt br gt Background High-molecular-weight glutenin subunits HMW-GS play important roles in the elasticity of dough lt br gt made from wheat. The HMW-GS null line is useful for studying the contribution of HMW-GS to the end-use quality lt br gt of wheat. lt br gt Methods In a previous work we cloned the Glu-1Ebx gene from Thinopyrum bessarabicum and introduced it into lt br gt the wheat cultivar Bobwhite. In addition to lines expressing the Glu-1Ebx gene we also obtained a transgenic line lt br gt LH-11 with all the HMW-GS genes silenced. The HMW-GS deletion was stably inherited as a dominant and conformed lt br gt to Mendel s laws. Expression levels of HMW-GS were determined by RT-PCR and epigenetic changes in methylation lt br gt patterns and small RNAs were analyzed. Glutenins and gliadins were separated and quantitated by reversed-phase lt br gt ultra-performance liquid chromatography. Measurement of glutenin macropolymer and analysis of agronomic traits lt br gt and end-use quality were also performed. lt br gt Results DNA methylation and the presence of small double-stranded RNA may be the causes of post-transcriptional lt br gt gene

• Sinh học
• BMC Plant Biology
• Common wheat
• High molecular weight glutenin subunits
• Post transcriptional gene silencing
• Glutenin macropolymer
• Gliadin content
• Single plant genome wide association study
• Plant height
• Bulk segregant analysis
• Genome wide association studies
• Virus vector host interaction
• Plant defense
• Jasmonic acid
• Plant volatile
• Bemisia tabaci
• Terpenoid indole alkaloid
• Madagascar periwinkle
• Transcription factors
• Plant secondary metabolism
• High throughput sequencing
• Plant stress response
• Chemical library
• Pollen tube
• Growth regulator
• Modulate plant growth
• Community ecology
• Competitive exclusion
• Latitudinal gradients
• Plant–fungus interactions
• Plant–soil feedback
• Biodiversity effects
• Experimental grassland
• Herbivore induced plant volatiles
• Volatile organic compounds
• Herbivore induced plant volatiles
• Solidago altissima
• Eurosta solidaginis
• Plant physiology
• Auxin quantification
• Isotope dilution analysis
• Laser microdissection microscope
• Plant sample preparation
• Minute samples
• Linkage map
• Technical length
• Quantitative trait locus
• Pitcher plant
• DNA barcoding
• Cytochrome oxidase I
• Nepenthes genus
• Bivariate quantiles
• Conditional quantiles
• Joint estimation
• Plant growth
• Stress tolerance
• Mepiquat chloride
• Lateral root
• Plant growth regulator
• Enhances root growth
• Ammonium metabolism
• Arabidopsis thaliana
• Plant defensins
• Amino acids
• Genome wide association study
• Lint percentage
• Boll number per plant
• Boll weight per plant
• Salt stress
• Transcriptome analysis
• Gossypium hirsutum
• Catalpa bungei
• Genetic map
• RAD seq
• QTL mapping
• Leaf traits
• HN09 induced systemic resistance
• Plant immune
• Plant growth promoting rhizobacteria
• Bacillus subtilis HN09
• Plant architecture
• RNA Seq
• Internode elongation
• Plant hormone
• Physic nut
• Arabidopsis TFL1
• Mutants reveals cytokinin
• Woody plant Jatropha curcas
• Sucrose transporters
• Abiotic stress
• ABA signaling pathway
• Stomatal closure
• Tomato plant
• Pathogenesis related gene 1
• Regulatory mechanism
• Transcription factor
• RNA polymerase III
• Plant specific zinc dependent
• DNA binding proteins
• Meta transcriptomics
• Plant abiotic stress
• Plant biotic stress
• Crop physiology
• ABA hypersensitive
• Plant biological
• Including phytohormone homeostasis
• Plant development
• Photosynthetic plant life
• Plants perceive UV B
• Non plant organisms
• Guanine nucleotide exchange factors
• Chromatin immunoprecipitation
• Colletotrichum gloeosporioides
• Induced response
• Plant–pathogen interaction
• Polyphenol oxidase