TAILIEUCHUNG - Growth of marine fungi on polymeric substrates

Marine fungi are a diverse group of opportunistic and obligate organisms isolated from marine environments. These fungi are now often included in screens for novel metabolites, while less attention has been given to their production of hydrolytic enzymes. | Wang et al. BMC Biotechnology 2016 16 3 DOI S12896-016-0233-5 BMC Biotechnology RESEARCH ARTICLE Open Access z . I r r I CrossMark Growth of marine fungi on polymeric substrates Yanming Wang Dorothee Barth Anu Tamminen and Marilyn G. Wiebe Abstract Background Marine fungi are a diverse group of opportunistic and obligate organisms isolated from marine environments. These fungi are now often included in screens for novel metabolites while less attention has been given to their production of hydrolytic enzymes. Most enzymes derived from marine microorganisms have been obtained from marine bacteria. The enzymes produced by marine fungi may have different properties than those derived from bacteria or from terrestrial fungi. Here we assess the growth of six filamentous marine fungi on a wide range of polymeric substrates as an indication of their general capacity to produce hydrolytic enzymes. Results Calcarisporium sp. KF525 Tritirachium sp. LF562 Bartalinia robillardoides LF550 Penicillium pinophilum LF458 Scopulariopsis brevicaulis LF580 and Pestalotiopsis sp. KF079 all grew on both casein and gelatin as N-source indicating secretion of proteases. All species also grew on starch laminarin xylan pectin and oil indicating production of amylases glucanases xylanases pectinases and lipases. Growth on cellulose occurred but was weaker than on xylan. All strains also grew to some extent on sulphated arabinogalactan although only LF562 could utilise arabinose. Four strains grew on the sulphated ulvans whereas only KF525 grew on agar or carrageenan. KF525 and LF562 showed limited growth on alginate. Although fucose was used as carbon source by several species fucoidan did not support biomass production. Conclusions Marine fungi could be excellent sources of a wide range of hydrolytic enzymes including those able to hydrolyse various seaweed polymers. Although the native hosts may secrete only small amounts of these enzymes the genes may provide a rich source of .

• Sinh học
• BMC Biotechnology
• Marine fungi
• Hydrolytic enzymes
• Radial growth rate
• Bartalinia robillardoides LF550
• Penicillium pinophilum LF458
• Transgenic animals
• Recombinant proteins
• Animal biotechnology
• Functional cytokines
• ABC transporter
• Taxonomic diversity
• ATP binding cassette
• Transporter gene superfamily
• Community engagement
• Agricultural biotechnology
• Tissue culture
• Global health
• Global development
• Stakeholder engagement
• BMC Chemistry
• Citric acid
• White biotechnology
• Organic acid
• Emerging bioeconomy
• MicroRNAs
• cDNA synthesis
• synthetic templates
• Design of PCR primers
• Quantitative PCR
• Error prone PCR
• Site directed mutation
• Site saturation mutagenesis
• Catalytic efficiency
• Tobacco stalk
• Nicotine degradation
• Phanerochaete chrysosporium
• Lignocellulolytic enzymes
• Chaetomium globosum
• Synergistic action
• Lignocellulosic materials
• Phenolic alcohols
• β xylosidase
• Highly thermostable GH39
• Geobacillus sp
• Hydrolyzing xylo oligosaccharides
• Heterologous expression
• Kluyveromyces lactis
• Baking industry
• Plectosphaerella cucumerina
• Gelatin scaffold
• Human articular chondrocytes
• Extracellular matrix
• Cartilage tissue engineering
• Protein design
• Protein expression
• Protein interaction
• Protein engineering
• Pseudomonas putida strains
• Raw glycerol
• PHA depolymerase
• Metabolic engineering
• Protein cross linking
• Protein stability
• Baculovirus expression vector system
• Human influenza strain
• Arteriovenous loop
• PHD inhibitor
• Dimethyloxallyl Glycine
• HIF α level
• Substitution pattern
• Hydroxypropyl methylcellulose
• Application of carbohydrate binding modules
• Hydroxyethyl cellulose
• Rhizomucor pusillus
• α amylase
• Co expression
• Pichia pastoris
• Alkaline phosphatase
• Escherichia coli
• Psychrophilic alkaline phosphatase
• Hydrolytic cleavage
• Leuconostoc citreum
• Inulosucrase IslA
• Multidomain fructosyltransferase
• Multidomain enzyme
• Hydrolytic activity
• CD spectroscopy
• Homology modelling
• Peroxidase activity
• Superoxide dismutase
• Trichoderma reesei
• Endoglucanase I
• Cell free synthesis
• Saccharomyces cerevisiae
• Neurospora crassa
• Glutaminyl cylcase
• Tobacco mosaic virus
• Protein purification
• N benthamiana
• Active pharmaceutical ingredient
• Adenoviral vector
• Inducible vector
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• Co transformation
• Gaussia princeps luciferase gene
• Genetic engineering
• Green algae
• Inducible promoter
• Nitrate reductase
• Spider silk
• Synthetic spidroin
• Atomic force microscopy
• Outstanding mechanical