TAILIEUCHUNG - Expression of GATA4 gene into differentiated canine induced pluripotent stem cell

Induced Pluripotent stem cells (iPSC) technology is involved in advancing the field of disease modelling and cell transplantation. Stem cell can differentiate under appropriate in vitro and in vivo conditions into different cell types. Under this study, we analysed the establishment of in vitro expression of GATA4 gene in cardiac differentiated canine iPSC. In vitro differentiation of canine iPSCs via embryoid bodies (EBs) was produced by ‘Hanging Drop’ method. EB’s were differentiated by using cardiac differentiation media. During differentiation, EBs was collected on day 4, 6, 8, 12, 16, 20 and 24 for characterization of cardiomyocytes specific marker expression. Total c by using the Trizol method and subsequently, cDNA was synthesized. The differentiated cells expressed cardiac-specific gene GATA 4 which started from day 4 of differentiation. Relative expression of cardiac-specific genes revealed that ciPSC has the potential to differentiate into cardiomyocytes which can be used for cardiac tissue regeneration and as disease models for pharmaceutical testing. | Expression of GATA4 gene into differentiated canine induced pluripotent stem cell

• Sinh học
• Heart failure
• GATA4 gene into differentiated
• Canine induced pluripotent stem cell
• Models for pharmaceutical testing
• Models for pharmaceutical testing
• Drug in heart failure
• Device selection in heart failure
• The pathophysiology of heart failure
• Traditional mechanical support
• Inotropic therapy for heart failure
• Lecture heart failure
• Classification of heart failure
• The cause of heart failure
• Symptoms of heart failure
• Heart failure Definition
• Percutaneous mechanical support
• Cardiac resynchronization therapy
• Atrial arrhythmias in heart failure
• Heart transplantation
• Patients with chronic heart failure
• Chronic heart failure
• Self care among patients
• Patient’s knowledge about heart failure
• Physical activity of the patients
• BMC Genomics
• RNA seq
• Gene expression
• Current heart failure
• Etiology specific gene
• BMC Geriatrics
• Heart failure stages
• Population attributable risk
• All cause mortality
• Heart failure management
• The neural pathways
• Barorefl ex activation therapy in heart failure
• Modulation of autonomic function
• Therapies in heart failure
• Listening to the heart
• Nervous system regulation
• Cerebral aging
• The autonomic nervous system
• NT proBNP
• Premature ventricular complexes
• Stable ischemic heart disease
• Chronic ischemic heart disease
• Heart muscle disease
• Heart rhythm disorders
• Peripheral vascular disease
• Heart disease in the young
• Heart disease in the elderly
• Cardiovascular drugs
• Evaluation and management
• Right sided heart failure
• Right heart failure
• Main points of RHF
• Cardiac embryogenesis
• BMC Veterinary Research
• Platelet proteomic
• Myxomatous mitral valve disease
• Congestive heart failure
• knowledge of medicine
• pharmacy management
• medication management
• vasodilators
• Extracorporeal membrane oxygenation
• Pathophysiology of heart failure
• Pediatric patients
• Respiratory failure
• Diastolic heart failure
• Cardiogenic shock
• Cardiovascular diseases
• Ischemic Heart Disease
• Stable ischemic heart disease
• Acute coronary syndromes
• Cardiac arrhythmias
• Ischemic stroke
• MCQs for cardiology knowledge based assessment
• Cardiology knowledge based assessment
• Ischaemic heart disease
• Valvular heart disease
• Adult congenital heart disease
• Pathophysiology of heart diseas
• The cardiomyopathies
• Mechanisms of cardiac arrhythmias
• Clinical aspects of cardiac arrhythmias
• Congenital heart disease
• Diseases of the pericardium
• Cardiology clinical questions
• Cardiology clinical
• Congenital heart diseases
• Heart failure and hypertension
• Managing chronic heart failure patient
• Chronic kidney disease
• Modification of risk factors
• Quản lý bệnh nhân suy tim mãn tính
• Bệnh thận mãn tính
• Điều trị bệnh thận
• Study of clinical characteristic
• Risk factors
• Deep vein thrombosis
• Lower extremities
• Pulmonary emboli
• Practical cardiovascular medicine
• Non‐ST‐Segment elevation acute coronary syndrome
• ST‐segment elevation myocardial infarction
• Additional heart failure topics
• Valvular disorders
• Left ventricular filling pressure
• Diastolic dysfunction
• Echocardiography guidelines
• Doppler echocardiography
• Renal dysfunction
• Estimated glomerular filtration rate
• Left ventricular ejection fraction
• Serum BNP
• Heart failure with preserved ejection fraction
• Narrow QRS complex
• Speckle tracking echocardiography
• Dyssynchrony remains unclear
• QRS complex