TAILIEUCHUNG - Characterization of rice polyphenol oxidase promoter in transgenic Arabidopsis thaliana

Polyphenol oxidase (PPO) enzymes are ubiquitous in plant kingdom and catalyze the oxidation of phenols to highly reactive quinones. The PPO gene plays an important role in plant defense mechanisms against biotic and abiotic stresses but the regulation of PPO promoter in stresses remains unclear. | Turkish Journal of Botany Turk J Bot (2017) 41: 223-233 © TÜBİTAK doi: Research Article Characterization of rice polyphenol oxidase promoter in transgenic Arabidopsis thaliana 1 2 3 2, Wasim AKHTAR , Ejaz AZIZ , Hisashi KOIWA , Tariq MAHMOOD * Department of Biotechnology, University of Azad Jammu and Kashmir, Muzaffrabad, Pakistan 2 Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan 3 Department of Horticultural Sciences, Texas A&M University, College Station, TX, USA 1 Received: Accepted/Published Online: Final Version: Abstract: Polyphenol oxidase (PPO) enzymes are ubiquitous in plant kingdom and catalyze the oxidation of phenols to highly reactive quinones. The PPO gene plays an important role in plant defense mechanisms against biotic and abiotic stresses but the regulation of PPO promoter in stresses remains unclear. Here Oryza sativa (OsPPO) promoter was fused to firefly Luciferase (LUC) and β-glucuronidase (GUS) reporter genes separately to test the effects of wounding, abscisic acid (ABA), and methyl jasmonate (MeJ) applications on PPO promoter induction. To the best of our knowledge, this is the first report about rice PPO promoter induction in response to wounding and ABA characterized by RT-PCR. Transcriptional profiling of reporter genes by real-time (RT) PCR in transgenic Arabidopsis revealed ~ induction under PPO promoter directed activity in response to ABA treatment. Moreover, a strong induction of 11-fold was observed in response to wounding in reporter gene transcripts under control of OsPPO promoter. Plant cis-acting regulatory DNA elements signal scanning of OsPPO promoter also showed homologies to ABA cis-regulatory element signaling complexes and wound responsive elements (W-boxes) residing within the 1020 bp promoter region. Wound and ABA inducibility of OsPPO promoter is a strong indicator of its role in the

• Nông nghiệp
• Wound induction
• Transgenic Arabidopsis
• Polyphenol oxidase
• Plant kingdom
• Abiotic stresses
• Abscisic acid
• Agricultural science
• Dichlorophenoxy acetic acid
• Hydrogen peroxide induced resistance related
• Components against potato early blight
• động vật
• thực vật
• khí khổng
• thoat hơi nước
• Bài giảng Sinh lý thực vật
• Khám phá Abscisic Acid
• Sản phẩm quang hợp
• Quá trình phát sinh phôi
• Sinh tổng hợp
• Trao đổi ABA ở thực vật
• Citrullus lanatus
• Chilling injury
• Root fresh
• Growing watermelons
• Phanerochaete chrysosporium ME446
• Immobilization cells
• Polyurethane foam
• Synthesize auxin
• BMC Plant Biology
• Galactinol synthases
• Water deficit
• Biotic stress tolerance
• Chinese jujube
• Ziziphus jujuba
• Fruit ripening
• Non climacteric
• Transcriptome sequencing
• Flame seedless
• Fruit quality
• Time of ripening
• Fruit quality of flame seedless grape
• Pre harvest application of hydrogen cyanamide
• Plant Biology
• RNA Seq
• Transcription factors
• Resistance genes
• BMC Genomics
• Hordeum vulgare
• Sucrose nonfermenting 1
• Gene family
• Metabolic regulation
• Stress responses
• Gerbera hybrida
• Stem bending
• Water stress
• Oryza sativa L
• Grain yield and quality
• Assimilates allocation
• Sugar metabolism
• Abscisic acid signaling
• Organ specificity
• Vitis vinifera L
• BiNGO plug in software
• Proline metabolism
• Root oxidative damage
• Hypoxic stress
• Gibberellic acid
• Jasmonic acid
• Salicylic acid
• 5 Aminolevulinic acid dehydratase
• Growth criteria
• Corn Zea mays L
• Corn seedlings
• Berry set
• Indole 3 acetic acid
• Gas chromatography
• Drought stress
• Transcription factor
• Drought responsive gene
• Senescenceassociated gene
• Arabidopsis thaliana
• Verticillium longisporum
• Oilseed rape
• Quantitative disease resistance
• Lentinus tigrinus
• Laetiporus sulphureus
• Olive mill waste water
• Plant hormones
• Chilling stress
• Nitric oxide
• Oxidative damage
• Antioxidant enzyme
• Transgenic tobacco
• Phosphatidic acid
• Stomatal closure
• Lipid peroxidation
• Phosphate starvation response
• Ubiquitin proteasome pathway
• P coumaric acid
• Rourea harmandiana
• Ethyl acetate extract
• Chemical constituents of Rourea Harmandiana Pierre
• Abiotic stress
• Biotic stress