TAILIEUCHUNG - Molecular characterisation of the genus Papaver section Oxytona using ISSR markers

Papaver bracteatum Lindl., P. orientale L., and P. pseudo-orientale (Fedde) Medw., species of section Oxytona Bernh. in the genus Papaver L., are found widely in the native flora of Turkey. They are commercially important for their high alkaloid content. | Turkish Journal of Botany Turk J Bot (2013) 37: 644-650 © TÜBİTAK doi: Research Article Molecular characterisation of the genus Papaver section Oxytona using ISSR markers 1 1 2 1 3, Tuğba GÜRKÖK , Elif KAYMAK , Gülşen BOZTEPE , Mesut KOYUNCU , İskender PARMAKSIZ * 1 Department of Biology, Faculty of Arts and Sciences, Gaziosmanpaşa University, Tokat, Turkey 2 Tunceli University Vocational School, Tunceli, Turkey 3 Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Gaziosmanpaşa University, Tokat, Turkey Received: Accepted: Published Online: Printed: Abstract: Papaver bracteatum Lindl., P. orientale L., and P. pseudo-orientale (Fedde) Medw., species of section Oxytona Bernh. in the genus Papaver L., are found widely in the native flora of Turkey. They are commercially important for their high alkaloid content. Because genus Papaver section Oxytona has similar morphological characters, it is not easy to distinguish these species. We used the inter-simple sequence repeat (ISSR) molecular marker system to determine the molecular characterisation of this section. In this study, 20 ISSR primers have been studied in 180 accessions collected from 5 different regions of Turkey. A total of 82 bands were obtained, of which 80 were polymorphic. Average genetic distance was found to be , the Shannon index was , and the polymorphism rate was . The primer AT10 had the smallest number of bands, which was 3, while the primers AT19 and AT3 had the greatest number of bands, which was 5. In conclusion, the ISSR marker system can be used for the classification of section Oxytona. Key words: Papaver, Oxytona, ISSR, molecular marker, phylogeny 1. Introduction Turkey is one of the gene origins for many plants, including Papaver L. (Davis et al., 1988; Atalay, 1994; Erik & Tarıkahya, 2004). The species belonging to Papaveraceae are .

• Nông nghiệp
• Papaver bracteatum
• Genus Papaver
• Papaver section Oxytona
• Oxytona Bernh
• Inter simple sequence repeat
• Cell suspension culture
• Secondary metabolites
• Iranian poppy
• Dry cell weight
• Poa angustifolia
• Poa trivialis
• Intersimple sequence repeat
• Genetic relationship
• Genetic diversity
• Genus Onobrychis Miller
• Onobrychis species
• Genetic similarity
• Principal coordinates analysis
• Inula tuzgoluensis
• Scanning electron microscopy
• I
• aucheriana
• Pollen grains
• Pollen characteristics
• Population genetics
• Species complex
• Mexican Volcanic Belt
• Plant morphology
• Cluster analysis
• Endangered medicinal plant
• Molecular marker
• Evaluation of genetic diversity
• Barley Hordeum spp
• Barley leaf stripe
• Genetic resistance
• Restriction fragment length polymorphism