TAILIEUCHUNG - Identification of HLA-A*0201-restricted CTL epitopes from the receptor-binding domain of MERS-CoV spike protein using a combinatorial in silico approach

A novel SARS-like illness called Middle East respiratory syndrome coronavirus (MERS-CoV), caused by an emerging coronavirus, has been a recent cause for concern due to its fatality and pandemic potential. Developing a peptide-based vaccine could be helpful in fighting against the virus. | Turkish Journal of Biology Research Article Turk J Biol (2014) 38: 628-632 © TÜBİTAK doi: Identification of HLA-A*0201-restricted CTL epitopes from the receptor-binding domain of MERS-CoV spike protein using a combinatorial in silico approach Naghmeh POORINMOHAMMAD, Hassan MOHABATKAR* Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran Received: Accepted: Published Online: Printed: Abstract: A novel SARS-like illness called Middle East respiratory syndrome coronavirus (MERS-CoV), caused by an emerging coronavirus, has been a recent cause for concern due to its fatality and pandemic potential. Developing a peptide-based vaccine could be helpful in fighting against the virus. Since the experimental procedure is time-consuming and expensive, computational analysis can play an important role in accelerating the process. Therefore, the aim of this study was to computationally identify cytotoxic T-lymphocyte epitopes presented by the human leukocyte antigen (HLA)-A*0201, as this is the most frequent HLA class I allele among Middle Eastern populations. The receptor-binding domain of the spike glycoprotein of MERS-CoV, by which the virus binds to its entry receptor to further infect host cells, is a potential candidate used here for running our in silico epitope identification process. The results include predicted epitopes together with their interaction properties with major histocompatibility complex (MHC) molecules and also the binding behavior of MHC–epitope complexes to human T-cell receptor. Predicted epitopes with the most preferable binding properties are beneficial for vaccine development. Therefore, the huge experimental workload for epitope-based vaccine design will be minimized. Key words: Middle East respiratory syndrome coronavirus, cytotoxic T-lymphocyte epitopes, HLA-A2, computational .

• Sinh học
• Middle East respiratory syndrome coronavirus
• Cytotoxic T lymphocyte epitopes
• Computational prediction
• Vaccine design
• Major histocompatibility complex
• BMC Anesthesiology
• Convalescent plasma
• Randomized controlled trial
• Randomized controlled trials
• BMC Pulmonary Medicine
• Nosocomial infection
• Infection control
• Hand hygiene
• BMC Bioinformatics
• Binding affinity prediction
• Peptide data
• Recurrent neural networks
• Language modeling
• Machine learning
• Antigen presentation
• Random forest
• Major histocompatibility complex class I
• BMC Genomics
• Family Equidae
• MHC class II loci
• Positive selection
• Trans species polymorphism
• Genome Biology
• Human leukocyte antigen
• Major risk factors
• Including large genetic screens
• Different diseases
• báo cáo hóa học
• công trình nghiên cứu về hóa học
• tài liệu về hóa học
• cách trình bày báo cáo
• Báo cáo sinh học hay
• báo cáo sinh học
• công trình nghiên cứu sinh học
• tài liệu về sinh học
• tài liệu báo cáo nghiên cứu khoa học
• kiến thức bệnh thú y
• báo cáo bệnh thú y
• các nghiên về bệnh thú ý
• trình bày báo cáo
• tài liệu báo cáo khoa học
• cáo cáo y học
• nghiên cứu y học
• kiến thức y học
• Haplotype block
• Linkage disequilibrium
• Rheumatoid arthritis
• Single nucleotide polymorphism
• BMC Cancer
• Cancer stem cells
• Cancer initiating cells
• Animal models
• Lung cancer
• Major histocompatibility complex I
• Cytotoxic T lymphocytes
• HLA imputation software
• Targeted therapy
• Peptide HLA
• Cell surface
• Cancer cells
• BMC Molecular and Cell Biology
• Quality control
• Intracellular trafficking
• Protein sorting
• Rapamycin trapping assay
• Addax nasomaculatus
• Physical map
• Immunological defence
• Journal of Chemistry
• Tumour necrosis factor
• Square wave voltammetry
• Genome wide association
• Electrochemical measurements
• Balancing selection maintains variation
• Brassicaceae species
• Phenotypic differences
• BMC Genetics
• Novel alleles
• Polymorphic loci
• Cytotoxic immune response
• Rhodeus sinensis
• Balancing selection
• Odocoileus virginianus
• Chromosomal inversion
• Usmle road map Immunology
• Innate immunity
• Adaptive immunity
• Antigens and antibodies
• Immunoglobulin gene expression
• Antigen recognition by antibody
• Kuby immunology
• The immune system
• Receptors and signaling
• The complement system
• The major histocompatibility complex