TAILIEUCHUNG - Random amplified polymorphic DNA as a method to screen metal-tolerant barley (Hordeum vulgare L.) genotypes

Differentially responding genotypes are comparatively used to understand mechanisms of metal tolerance in higher plants. The feasibility of the random amplified polymorphic DNA (RAPD) technique was evaluated to screen for metal tolerance within plants as an alternative to physiological growth parameters. | Turkish Journal of Botany Research Article Turk J Bot (2015) 39: 747-756 © TÜBİTAK doi: Random amplified polymorphic DNA as a method to screen metal-tolerant barley (Hordeum vulgare L.) genotypes Süleyman CENKCİ*, Nevra DOĞAN Department of Molecular Biology and Genetics, ANS Campus, Afyon Kocatepe University, Afyonkarahisar, Turkey Received: Accepted/Published Online: Printed: Abstract: Differentially responding genotypes are comparatively used to understand mechanisms of metal tolerance in higher plants. The feasibility of the random amplified polymorphic DNA (RAPD) technique was evaluated to screen for metal tolerance within plants as an alternative to physiological growth parameters. Eight hydroponically grown barley (Hordeum vulgare L.) genotypes were exposed to different concentrations (75, 150, and 225 µM) of cadmium and copper for 7 days and subsequently harvested for determining metal tolerance indices by comparing dry biomass to genomic template stabilities based on their RAPD profiles. Dose- and genotypedependent reductions in dry weights of shoot and root tissues were detected for both metal treatments. The tolerance indices showed that Aydanhanım/Bülbül-89 and Başgül/Tarm-92 were the most tolerant/sensitive barley genotypes to cadmium and copper, respectively. For untreated barley genotypes, the amplified RAPD bands were genotype-dependent and estimated genetic similarity coefficients ranged from to . Metal exposure caused appearance and/or disappearance of RAPD bands in comparison to the control RAPD profiles. The alterations in RAPD profiles were dose- and genotype-dependent for the cadmium and copper treatment groups. Consequently, genomic template stability (GTS, %) of all barley genotypes was reduced with metal exposure. Based on GTS values, the most tolerant/ sensitive barley genotypes to cadmium and copper were Aydanhanım/Tarm-92 and .

• Nông nghiệp
• Genomic template stability
• Tolerance index
• Random amplified polymorphic DNA
• Hordeum vulgare L
• Polymerase chain reaction
• Antioxidant enzymes
• Solanum lycopersicum L
• Relative water content
• Malondialdehyde content
• Bài giảng Polymerase Chain Reaction
• Taq polymerase
• Enzyme bền nhiệt
• Thiết kế primer
• PCR cải biến
• Bombyx mori L
• Grasserie virus
• Detection of grasserie virus
• Bovine papilloma
• Polymerase chain reaction in cattle
• Detection of bovine papillomavirus
• Bovine papillomavirus in cutaneous lesions
• Medicinal and aromatic plants
• Rapid DNA isolation
• Secondary metabolites
• Subsequent polymerase chain reaction
• L monocytogenes
• Chicken meat
• Areas of chennai
• Microbial quality (Listeria monocytogenes)
• Chicken meat by polymerase chain reaction
• Diagnosis of equine herpes virus
• Equine herpes virus 4 infection
• Horses showing symptoms of respiratory disease
• Tạp chí y học
• Nghiên cứu y học
• Mycobacterium tuberculosis và mycobacterirum khác mycobacterium tuberculosis
• Bệnh lý lao da
• Phản ứng chuỗi polymerase
• kỹ thuật PCR
• DNA
• phương pháp khuếch đại đoạn DNA
• nghiên cứu sinh học
• sinh học
• bài giảng sinh học
• Luận văn Thạc sĩ Sinh học
• Ứng dụng phương pháp PCR
• Sự nhiễm vi sinh vật gây bệnh
• Thực phẩm đường phố
• VSV gây bênh trong TP đường phố
• C perfringens trong thực phẩm đường phố
• certification of conventional soybean seeds and grains
• detection of transgenic soybean
• polymerase chain reaction (PCR)
• Roundup Ready™ (RR™) soybean
• Virus cúm gia cầm A H5N1
• Kỹ thuật Multiplex Reverse Transcription Polymerase Chain Reaction
• Luận văn thạc sĩ khoa học
• Virus cúm A
• Sinh học thực nghiệm
• nhuộm Haematoxylin và Eosin
• luận văn thủy sản
• nuôi trồng thuỷ sản
• giải pháp phát triển
• ngành thủy sản
• chuyên đề thực tập
• Tạp chí khoa học
• Tạp chí y dược
• Y dược quân sự
• Kỹ thuật reverse transcription polymerase chain reaction
• Xác định virut Ebola
• Định lượng hepatitis B virus DNA
• Kỹ thuật real time polymerase chain reaction
• Nhiễm virus viêm gan B
• Týp gen caga và vaca
• Phương pháp multiplex polymerase chain reaction
• Viêm dạ dày
• Bacterial vaginosis
• Multiplex polymerase chain reaction
• Bacterial vaginosis detection
• Serious complications affecting
• Long term health of women
• BMC Pediatrics
• HIV 1 infection
• Filter paper dried blood spots
• HIV 1 diagnosis
• Early diagnosis
• Early diagnosis of leptospirosis
• Clinical diagnosis
• Leptospirosis during
• Presence of campylobacter jejuni
• Cross contamination
• Food borne bacterial illness
• Antibiotic treatment
• Rapid enterovirus polymerase chain reaction
• Aseptic meningitis
• Causative viral infections
• Mycobacterium avium subspecies paratuberculosis
• Nested PCR
• ZiehlNeelsen staining
• Nested polymerase chain reaction
• Diagnosis of paratuberculosis
• Theileria annulata
• Cattle calves
• Cross bred
• Breed related hospital prevalence
• Theileria annulata infection in cattle calves confirmed
• Enzyme linked immunosorbent assay
• CMV DNA
• Pregnant women
• Detection of cytomegalovirus
• PALCAM selective agar
• BHI brothand PCR
• Molecular polymerase chain reaction
• Employing conventional isolation techniques
• Klebsiella oxytoca
• Molecular diagnosis
• Polymerase chain reaction targeted
• polygalacturonase gene
• Molecular discrimination of klebsiella oxytoca