TAILIEUCHUNG - cDNA cloning, molecular characterization, and expression analyses of two novel porcine ARRDC genes-ARRDC1 and ARRDC5

Arrestin-domain–containing proteins belong to the arrestin family, members of which are important for regulating signal transduction within cells. In this study, two ARRDC genes (ARRDC1 and ARRDC5) of Diannan small-ear pigs were amplified by reverse transcription polymerase chain reaction and cloned for the first time (accession nos.: KF589202 and KF589203). | Turkish Journal of Biology Turk J Biol (2015) 39: 523-531 © TÜBİTAK doi: Research Article cDNA cloning, molecular characterization, and expression analyses of two novel porcine ARRDC genes—ARRDC1 and ARRDC5 1,2, 1,3,4, 3,4 4 4 5 Hailong HUO *, Pei WANG *, Shuyan WANG , Yongwang MIAO , Hongjiang WEI , Yongyun ZHANG , 6 7 2,4 2 4 2 3,4 1, , Qiaoling ZHANG , Fuquan LI , Lixian LIU , Rui WANG , Weizhen LI , Yue ZHAO , Jinlong HUO , Heng XIAO * ** 1 Faculty of Life Science, Yunnan University, Kunming, Yunnan, . China 2 Department of Husbandry and Veterinary, Yunnan Vocational and Technical College of Agriculture, Kunming, Yunnan, . China 3 Key Laboratory of Banna Mini-Pig Inbred Line of Yunnan Province, Kunming, Yunnan, . China 4 Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, . China 5 Teaching Demonstration Center of the Basic Experiments of Agricultural Majors, Yunnan Agricultural University, Kunming, Yunnan, . China 6 College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin, . China 7 Department of Biotechnology, Neijiang Vocational and Technical College, Neijiang, Sichuan, . China Received: Accepted/Published Online: Printed: Abstract: Arrestin-domain–containing proteins belong to the arrestin family, members of which are important for regulating signal transduction within cells. In this study, two ARRDC genes (ARRDC1 and ARRDC5) of Diannan small-ear pigs were amplified by reverse transcription polymerase chain reaction and cloned for the first time (accession nos.: KF589202 and KF589203). The full-length coding sequence of ARRDC1 was 1284 nucleotides in length (molecular weight, kDa; pI, ) and encoded 427 amino acids. The ARRDC5 CDS was 1014 nucleotides in length (molecular weight, kDa; pI, ) and encoded 337 amino acids. Sequence homology .

• Sinh học
• Diannan small ear pig
• Arrestin domain containing 1
• Arrestin domain containing 5
• Arrestin family
• Tissue expression profile
• AKT genes
• Buffalo genes
• Protein kinase B
• CDNA synthesis
• Plant Biology
• Poplar bZIP gene family
• Tissue differential expression
• Salt stress
• Co expression analysis
• BMC Genomics
• Gene expression
• Amino acids
• Mitochondrial dysfunction
• Major endocrine organ