TAILIEUCHUNG - An improved PVS2 cryopreservation technique for Ascocenda Wangsa Gold orchid using protocorm-like bodies

Ascocenda Wangsa Gold is a new and fascinating orchid hybrid in the Malaysian flower industry. An efficient plant vitrification solution 2 (PVS2) cryopreservation technique was developed for protocorm-like bodies (PLBs) of Ascocenda Wangsa Gold orchid. | Turkish Journal of Biology Turk J Biol (2015) 39: 202-209 © TÜBİTAK doi: Research Article An improved PVS2 cryopreservation technique for Ascocenda Wangsa Gold orchid using protocorm-like bodies 1 1 1 Arulvilee RAJASEGAR , Asyraf MANSOR , Ranjetta POOBATHY , 2 3 1, Elayabalan SIVALINGAM , Uma Rani SINNIAH , Sreeramanan SUBRAMANIAM * 1 School of Biological Sciences, Universiti Sains Malaysia, Georgetown, Penang, Malaysia, 2 Department of Plant Molecular Biology and Biotechnology, Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India. 3 Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia Received: Accepted: Published Online: Printed: Abstract: Ascocenda Wangsa Gold is a new and fascinating orchid hybrid in the Malaysian flower industry. An efficient plant vitrification solution 2 (PVS2) cryopreservation technique was developed for protocorm-like bodies (PLBs) of Ascocenda Wangsa Gold orchid. Parameters assessed included the effect of PVS2 exposure periods, thawing duration, temperature, and culture conditions based on 2,3,5-triphenyltetrazolium chloride absorbance readings and regrowth rates. A regrowth rate of was obtained after 2 months when the PLBs were dehydrated in PVS2 for 30 min. The growth rate was improved to 47% when thawing was conducted at 45 °C for 85 s. The highest growth rate () was obtained when the PLBs were subjected to a 7-day dark treatment before being transferred to a 16-h/8-h light/dark photoperiod. Histological analyses were conducted to study the morphology of cryopreserved and noncryopreserved PLBs of Ascocenda Wangsa Gold. The vitrification protocol developed in this study is a feasible and safe method for strengthening the germplasm conservation of this orchid for commercial purposes. Key words: Plant vitrification solution .

• Sinh học
• Plant vitrification solution 2
• Liquid nitrogen
• Triphenyltetrazolium chloride assay
• Ascocenda Wangsa Gold
• Protocorm like bodies
• Dendrobium sonia 28
• Ascorbic acid
• Triphenyltetrazolium chloride