TAILIEUCHUNG - Collagen/PEO/gold nanofibrous matrices for skin tissue engineering

As a novel approach in skin tissue engineering, gold nanoparticles (AuNPs) were synthesized and incorporated at different concentrations into collagen/PEO nanofibrous matrices in this study. The group containing ppm AuNPs (CM-Au) had the best nanofibrous morphology. | Turkish Journal of Biology Turk J Biol (2016) 40: 380-398 © TÜBİTAK doi: Research Article Collagen/PEO/gold nanofibrous matrices for skin tissue engineering 1 1,2, Ömer AKTÜRK , Dilek KESKİN * Department of Engineering Sciences, Middle East Technical University, Ankara, Turkey 2 BIOMATEN, Center of Excellence in Biomaterials and Tissue Engineering, Middle East Technical University, Ankara, Turkey 1 Received: Accepted/Published Online: Final Version: Abstract: As a novel approach in skin tissue engineering, gold nanoparticles (AuNPs) were synthesized and incorporated at different concentrations into collagen/PEO nanofibrous matrices in this study. The group containing ppm AuNPs (CM-Au) had the best nanofibrous morphology. CM-Au was cross-linked with glutaraldehyde vapor (CM-AuX). All groups were disrupted in collagenase in 2 h, but cross-linked groups and Matriderm® resisted hydrolytic degradation for 7 and 14 days, respectively. Due to its small pores and dense structure, lower water swelling results ( ± g/g) were obtained for CM-AuX than Matriderm ( ± g/g). CM-Au and Matriderm had statistically similar tensile strength and elastic modulus, but elongation at break of CM-Au (over 100%) was significantly better than that of Matriderm. After cross-linking, tensile strength and elastic modulus of collagen matrix was further improved. AuNPs (37 and 42 nm) seemed to be nontoxic on 3T3 fibroblasts and keratinocytes for different time periods. CM-AuX scaffold extracts were also nontoxic for 3T3 fibroblasts and keratinocytes. The L929 cell attachment and proliferation on CM-AuX were comparable with Matriderm, indicating good in vitro biocompatibility. As a whole, collagen matrices incorporated with AuNPs are potential biomaterial candidates for skin tissue engineering. Key words: Nanofibrous matrix, electrospinning, collagen, gold nanoparticles 1.

• Sinh học
• Nanofibrous matrix
• Gold nanoparticles
• In vitro biocompatibility
• Nanofibrous morphology
• Skin tissue engineering
• BMC Molecular and Cell Biology
• Nanofibrous scaffold
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• Cardiomyocyte differentiation
• Extracellular matrix
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• ASTM D7136 tested
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• Alginate hydrogels
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