TAILIEUCHUNG - Biocompatible polymeric coatings do not inherently reduce the cytotoxicity of iron oxide nanoparticles
Nanotechnology in biomedical research is an emerging and promising tool for different purposes, like high-resolution medical imaging, diagnostics, and targeted drug delivery. Although some experimental efforts focused on determination of the cytotoxicity of nanoparticles (NPs) are present, there are many controversial results. | Turkish Journal of Biology Turk J Biol (2017) 41: 322-332 © TÜBİTAK doi: Research Article Biocompatible polymeric coatings do not inherently reduce the cytotoxicity of iron oxide nanoparticles 1,2,3, 2,3 1 4 Aylin ŞENDEMİR ÜRKMEZ *, Ece BAYIR , Eyüp BİLGİ , Mehmet Özgün ÖZEN 1 Department of Bioengineering, Faculty of Engineering, Ege University, İzmir, Turkey 2 Department of Biomedical Technologies, Graduate School of Natural and Applied Sciences, Ege University, İzmir, Turkey 3 Application and Research Center for Testing and Analysis (EGE-MATAL), Ege University, İzmir, Turkey 4 Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Department of Radiology, Canary Center at Stanford for Cancer Early Detection, Stanford University, Palo Alto, CA, USA Received: Accepted/Published Online: Final Version: Abstract: Nanotechnology in biomedical research is an emerging and promising tool for different purposes, like high-resolution medical imaging, diagnostics, and targeted drug delivery. Although some experimental efforts focused on determination of the cytotoxicity of nanoparticles (NPs) are present, there are many controversial results. In this study, chitosan (CS)- and poly(acrylic acid) (PAA)-coated iron oxide nanoparticles (IONPs) were used to investigate the possible cytotoxicity of coated IONPs on model mammalian cell line SaOs-2 by evaluating cellular viability and membrane integrity. Increasing concentrations of IONPs increased the cytotoxic effect on SaOs-2 cells with both CS- and PAA-coated IONPs. Cell viability on day 3 was as low as 40% and 48% at 1000 µM concentration for PAAand CS-coated IONPs, respectively, in 1% FBS-supplemented media. Cytotoxicity determined by the released lactate dehydrogenase (LDH) was as high as 163% with 1000 µM concentration of CS-IONPs, while there was no significant change in LDH release with PAAcoated IONPs at any concentration. .
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