TAILIEUCHUNG - Converted carotenoid production in Dunaliella salina by using cyclization inhibitors 2-methylimidazole and 3-amino-1,2,4-triazole

Carotenoids are vital for most photosynthetic organisms because of their crucial role in prevention of the damage caused by excess light or stress conditions like heat or nutrient deprivation. | Turkish Journal of Biology Research Article Turk J Biol (2017) 41: 213-219 © TÜBİTAK doi: Converted carotenoid production in Dunaliella salina by using cyclization inhibitors 2-methylimidazole and 3-amino-1,2,4-triazole Arzu YILDIRIM*, İsmail Hakkı AKGÜN, Meltem CONK DALAY Department of Bioengineering, Faculty of Engineering, Ege University, İzmir, Turkey Received: Accepted/Published Online: Final Version: Abstract: Carotenoids are vital for most photosynthetic organisms because of their crucial role in prevention of the damage caused by excess light or stress conditions like heat or nutrient deprivation. Some of them are also valuable for biotechnology wıth respect to their colorful feature and antioxidant properties. In this study, a natural β-carotene producer, green microalga Dunaliella salina, was evaluated for its potential to produce different valuable carotenoids through the inhibition of cyclization reactions in the carotenoid pathway by the chemical inhibitors 2-methylimidazole (2MI) and 3-amino-1,2,4-triazole (Amitrol). 2MI was shown to be effective on lutein accumulation in D. salina cultures grown at high temperature, without affecting cell viability. Addition of 2MI to the culture at 1 mM concentration resulted a increase in lutein content in the cell with the highest amount of pg/cell, and an associated decrease in β-carotene content suggesting that this inhibitor is more effective on lycopene beta-cyclase activity, thus shifting the pathway from β-carotene arm to the α-carotene direction. The results of this study may give the opportunity to use D. salina for the production of valuable carotenoids other than β-carotene. Key words: Dunaliella salina, carotenoids, chemical inhibitors, lutein 1. Introduction Carotenoids are the group of accessory pigments helping photosynthesis by absorbing light energy from sunlight at certain wavelengths.

• Sinh học
• Dunaliella salina
• Chemical inhibitors
• Photosynthetic organisms
• β carotene producer
• 2 methylimidazole
• Vi tảo lục đơn bào Dunaliella
• Sự tăng trưởng của Dunaliella salina
• Chủng Dunaliella salina
• Hình thái tế bào Dunaliella salina
• Khả năng chống oxy hóa của Dunaliella salina
• Seaweed liquid fertilizer
• Algal culture
• Quantity of biomass produced
• Micro algal culture
• Sự tăng trưởng Carotenoid
• tích lũy Carotenoid
• Tích lũy Lipid
• Tích lũy Carotenoid của Dunaliella salina
• Các điều kiện ức chế
• BMC Bioinformatics
• Metabolic network reconstruction
• Central carbon metabolism
• Flux balance analysis
• Tạp chí Khoa học
• Nuôi cấy vi tảo Dunaliella salina
• Quy mô pilot ở Việt Nam
• Vi tảo lục đơn bào
• Môi trường nuôi cấy RM2
• BMC Genomics
• Salt stress
• Transcriptomics analysis
• Third generation sequencing
• Secondgeneration sequencing
• Phương pháp keo tụ
• Keo tụ vi tảo Dunaliella salina
• Tế bào vi tảo
• Sinh vật đơn bào
• Phân bón NPK
• Vi tảo lục đơn bào Dunaliella salina
• Nuôi cấy vi tảo tăng trưởng
• Sắc tố và Beta caroten
• Tạp chí Đại học Sư phạm TP HCM
• Turkish Journal of Botany
• Tạp chí Thực vật học
• Seaweed based liquid
• Seaweed liquid fertilizers
• Nannochloropsis oculata
• Cost effective culture media
• Dunaliella salina hoạt tính sinh học
• Hoạt tính sinh học từ vi tảo
• Vi khuẩn lam Spirulina platensis
• Vi tảo Spirulina
• Sinh khối vi tảo
• Hiện tượng Đầm nước màu hồng
• Chất lượng môi trường nước
• Phiêu sinh thực vật
• Phương pháp buồng đếm
• Tảo lục Dunaliella salina