TAILIEUCHUNG - Results of the study on Naja siamensis antivenom purification in Vietnam and an assessment of safety and effective test in vitro and in vivo

The aim of this study is: To establish a protocol for NS antivenom purification and to assess the safety and efficacy in vitro and in vivo according to Vietnamese National Standards as well as WHO recommendation for antivenom products. The study contributed to resolve lack of NS antivenom in current treatment in Vietnam. | Journal of military pharmaco-medicine n06-2018 RESULTS OF THE STUDY ON Naja siamensis ANTIVENOM PURIFICATION IN VIETNAM AND AN ASSESSMENT OF SAFETY AND EFFECTIVE TESTS IN VITRO AND IN VIVO Le Khac Quyen*; Hoang Anh Tuan**; Thai Danh Tuyen***; Trinh Xuan Kiem** SUMMARY Objectives: To study Naja siamensis antivenom purification and assess safety and effective tests in vitro and in vivo. Methods: The study established a protocol for purification of F(ab’)2 Naja siamensis antivenom by using pepsin enzyme to cut Fc of IgG Naja siamensis horse antibody and removing complements and non-antibody components. Safety and efficacy tests were performed according to medium lethal dose (LD50) and medium effective dose (ED50). Results: This study produced successfully liquid and lyophilized Naja siamensis antivenoms accroding to Vietnamese National standardizations and WHO recommendations. An assessment of Naja siamensis antivenom in vitro and in vivo showed high safety and strong efficacy. Conclusion: The study on Naja siamensis antivenom F(ab')2 purification is successful. We hope that the study will spread out the trend of antidote treatment for Naja siamensis envenomed patients in future. * Keywords: Naja siamensis antivenom; Safety; Efficacy. INTRODUCTION In 2009, World Health Organization (WHO) classified snake bites as neglected tropical disease and reconfirmed that venomous snake antivenom is an antidote only of the treatment for envenomed patients by snake venom [9, 10]. Unavailable of specific antivenoms to treat the envenoming patients due to many venomous snake species in different areas in the world becomes a seriously medical important problem in the world [10]. From 1894, Dr. Calmette successfully researched to product cobra antivenom in Saigon Institute in the world [4]. As a result, treatment of snake envenoming became the new trend by using antidote of antivenom [4]. The study of Theakston showed an evidence-base for effectiveness of antivenom treatment based

• Y học thường thức
• Naja siamensis antivenom
• NS antivenom purification
• NS antivenom in current treatment
• Quality control of NS antivenom
• In vitro and in vivos animal model
• Potency test of NS antivenom