TAILIEUCHUNG - Influence of a low background radiation environment on biochemical and biological responses in V79 cells

Abstract We present the results of an experiment aimed at comparing the effects of different background radiation environments on metabolism and responses to γ-rays and cycloheximide of cultured mammalian cells. Chinese hamster V79 cells were maintained in exponential growth in parallel for up to 9 months at the Istituto Superiore di Sanità (ISS) and at the INFN-Gran Sasso underground Laboratory (LNGS) where exposure due to γ-rays and to radon was reduced by factors of about 70 and 25, respectively. | Radiat Environ Biophys (2002) 41:217–224 DOI O R I G I N A L PA P E R L. Satta · F. Antonelli · M. Belli · O. Sapora G. Simone · E. Sorrentino · . Tabocchini F. Amicarelli · C. Ara · . Cerù · S. Colafarina L. Conti Devirgiliis · A. De Marco · M. Balata A. Falgiani · S. Nisi Influence of a low background radiation environment on biochemical and biological responses in V79 cells Received: 14 December 2001 / Accepted: 1 June 2002 / Published online: 8 August 2002 © Springer-Verlag 2002 Abstract We present the results of an experiment aimed at comparing the effects of different background radiation environments on metabolism and responses to γ-rays and cycloheximide of cultured mammalian cells. Chinese hamster V79 cells were maintained in exponential growth in parallel for up to 9 months at the Istituto Superiore di Sanità (ISS) and at the INFN-Gran Sasso underground Laboratory (LNGS) where exposure due to γ-rays and to radon was reduced by factors of about 70 and 25, respectively. After 9 months the cells grown at the LNGS (cumulative γ dose about 30 µGy, average radon concentration around 5 Bq/m3), compared to the cells grown at the ISS (cumulative γ-ray dose about 2 mGy, average radon concentration around 120 Bq/m3), exhibited i) a significant increase of the cell density at confluence, ii) a significantly higher capacity to scavenge organic and inorganic hydroperoxides but a reduced scavenging capacity towards superoxide anions and iii) an increase in both the basal hprt mutation frequency and sensitivity to the mutagenic effect of γ-rays. The cells grown at the LNGS also showed a greater apoptotic sensitivity starting at the third month of culture, that was no longer detected after 9 months. Overall, these data L. Satta (✉) Energetics Department, Rome University “La Sapienza”, Via A. Scarpa 14, 00161 Rome Italy e-mail: Tel.: +39-064-9766560, Fax: +39-064-4240183 F. Antonelli · M. Belli · O. Sapora · G. .

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