TAILIEUCHUNG - Isolation and identification of chlorpyrifos degrading bacteria from rice upland crop soil in the mekong delta

Isolation and identification of chlorpyrifos degrading bacteria from rice upland crop soil in the mekong delta. The aim of this study is to isolate the bacterial strains which degrade Chlorpyrifos from rice-upland crop soils in the Mekong Delta. Twelve soil samples were taken at Cai Lay - Tien Giang, Cho Moi - An Giang and Binh Tan- Vinh Long. | No 6(31) – 2016, Dec. 2016 Scientific Journal Of Thu Dau Mot University ISOLATION AND IDENTIFICATION OF CHLORPYRIFOSDEGRADING BACTERIA FROM RICE-UPLAND CROP SOIL IN THE MEKONG DELTA Truong Quoc Tat, Duong Minh Vien Can Tho University ABSTRACT The aim of this study is to isolate the bacterial strains which degrade Chlorpyrifos from rice-upland crop soils in the Mekong Delta. Twelve soil samples were taken at Cai Lay - Tien Giang, Cho Moi - An Giang and Binh Tan-Vinh Long. Soil bacteria were enriched in mineral salt medium solution containing 20 mg/L of Chlorpyrifos as the only carbon source for bacterial growth. The results showed that one bacterial community was enriched and degraded Chlorpyrifos. One bacterial strain (coded as ) that was isolated from this community degraded % of Chlorpyrifos after 30 culture days. According to the sequencing of 16S rRNA gene, this bacterial strains was identified as Microbacterium sp. . Keyword: Bacteria, Chlorpyrifos, degradation, isolate 1. INTRODUCTION Application of the pesticide on agricultural crop is now a common practice and is an important factor of integrated pest management (IPM) strategies. It adversely affects the properties of the soil as well as it alters the pH of the soil required for microbial activities of beneficial bacteria to act upon [1], [2]. Some of these pesticides persist in the soil to form pollutants which may occasionally lead to surface and groundwater contamination. One of such pesticides is Chlorpyrifos. It is a broad-spectrum organophosphate insecticide, which is widely used to control insect pests on grain, cotton, fruit, nut, and vegetable crops, as well as lawns and ornamental plants in Viet Nam. The environmental fate of Chlorpyrifos has been studied extensively, and the reported half-life in soil varies from 10 to 120 days, with 3,5,6-trichloro-2pyridinol (TCP) as the major degradation product [3]. The manufacture and formulation process of Chlorpyrifos also generate waste

• Nông nghiệp
• Isolation and identification
• Chlorpyrifos degrading bacteria
• Rice upland crop soil
• The mekong delta
• The bacterial strains
• Escherichia coli infectio
• Poultry of south gujarat based
• Culture isolation
• Prevalence of Escherichia coli infection
• Identification of bacteria
• Subclinical mastitis
• Bacteria in subclinical mastitis
• Composition of buffalo milk
• Natural gas
• Single cell protein
• Identification of organisms
• Identification of fungi
• Soil in Loyola college campus
• Soil samples collected
• Loyola college campus
• Polymerase chain reaction
• Riemerella anatipestifer
• Ducks in assam
• Isolation and molecular identification
• Molecular identification
• Staphylococcus aureus
• Retail meat
• Frozen meat products and PCR
• Standard isolation techniques
• Detection of staphylococcus aureus
• Frozen chicken meat products
• Journal of Science and Technology
• Aerobic microbial community treating benzene
• Constructed wetland system
• Rhizosphere soil of phragmiteskarkas
• Vietnam Journal of Chemistry
• Isolation and identification of phenolic compounds
• The leaf extract of Cassia alata L
• The methanol leaf extract of C
• alata
• Partitioning the methanol extract
• Extended spectrum beta lactamases producing
• Klebsiella from human
• E coli
• Ceftazidime disk
• M tuberculosis
• Sheep tissue samples
• Sero diagnosis study
• Organized sheep farm
• Single spore
• Isolation of single spore
• Identification of resistance against soybean anthracnose
• Resistance against soybean anthracnose
• Soybean anthracnose
• Gram negative bacilli
• Non fermentative gram negative bacilli
• Contaminants or commensals
• Commonly used antibiotics
• Soil fungi of banana fields
• Baramati area
• Pune district of Maharashtra
• Investigation emphasizes
• Local isolate
• Several inoculum sources
• Rhizopus oligosporus local isolate derived
• Rhizopus oligosporus
• BMC Chemistry
• Macleaya microcarpa
• Biological activity
• Transgenic zebrafish
• Isolation of biological characteristics
• Identification of biological characteristics of porcine circovirus type 2
• Pigs in southern Vietnam
• Belonged to genotype PCV2b
• Porcine circovirus type 2
• Isolation of bioflocculant producing bacteria
• Identification of bioflocculant producing bacteria
• Cassava cultivated soil in Viet Nam
• Indole 3 acetic acid
• Micro organisms
• Soilborne fungi
• Harran plain
• Microfungal flora
• Acremonium Link ex Fr
• Floral characters
• Gamma radiation
• Gladiolus (Gladiolus grandiflorus L )
• Isolation of promising mutants
• vM2 generation
• Chicken meat
• PALCAM selective agar
• BHI brothand PCR
• Molecular polymerase chain reaction
• Employing conventional isolation techniques
• Lonar lake
• Phenol degradation
• Halomonas campisalis
• Phenol degrading halophilic bacteria
• Direct seeded rice
• Phyllosphere and methylobacterium
• Direct seeded rice growing areas
• PPFM bacterial isolates isolated
• Damping off
• Pure culture and PDA
• Onion (Alium cepa L
• ) plants
• Fungi causing damping off disease
• Genetic diversity within species of the genera Hanseniaspora
• Yeast strain
• Isolation of DNA for PCR ass
• RAPD PCR analysi
• PCR RFLP analysis of rDN