TAILIEUCHUNG - Properties of Bacillus cereus collected from different food sources

Forty-nine Bacillus cereus isolates were obtained from 10 different food samples consumed in Turkey. The isolates were identified as B. cereus based on colonial, cellular morphology, and biochemical characters, including fatty acid methyl ester (FAME) analysis. | Turk J Biol 33 (2009) 101-108 © TÜBİTAK doi: Properties of Bacillus cereus Collected from Different Food Sources Kıymet GÜVEN, M. Burçin MUTLU Department of Biology, Faculty of Science, Anadolu University, 26470 Eskişehir - TURKEY Received: Abstract: Forty-nine Bacillus cereus isolates were obtained from 10 different food samples consumed in Turkey. The isolates were identified as B. cereus based on colonial, cellular morphology, and biochemical characters, including fatty acid methyl ester (FAME) analysis. The potentials of 53 isolates including a reference strain, B. cereus NRRL B-3711, and 3 strains from Japan were tested to produce diarrhoeal enterotoxin (by the BCET-RPLA test) in BHI culture and 35 isolates (66%) were positive. Fatty acid composition varied for all B. cereus isolates, with 74 different fatty acids being detected. Grouping of B. cereus isolates by fatty acid composition suggested the existence of 4 groups: A, B, C, and D. The major difference between the groups was found in the following FAME: 15:0 ISO. Automated riboprinting was applied to the 53 isolates of B. cereus to assess the genetic similarity of the isolates obtained from different food sources. The automatic ribotyping of the total genomic DNA with EcoRI yielded a total of 8 band patterns among the isolates. Grouping of the 53 randomly selected B. cereus isolates based on their genotypic and phenotypic characteristics showed that these characteristics were independent of the food source of the various strains. The use of this polyphasic approach to strain differentiation enabled all the B. cereus isolates to be distinguished from each other. However, there was no relation between the groups and food of origin. Key Words: Bacillus cereus, food, diarrhoeal toxin, FAME, ribotyping Çeşitli Gıdalardan Toplanmış Bacillus cereus’ların Özellikleri Özet: Türkiye’de tüketilen 10 farklı gıda çeşidinden 49 Bacillus cereus izolatı toplanmıştır. İzolatlar koloni .

• Sinh học
• Bacillus cereus
• Diarrhoeal toxin
• Cellular morphology
• Biochemical characters
• Fatty acid methyl ester
• Gram staining
• Identification of Bacillus cereus
• Bacillus cereus from fish
• Voges proskauer test
• Đồ án tốt nghiệp
• Phát hiện nhanh Bacillus cereus
• Phát hiện nhanh Escherichia coli
• Sữa tiệt trùng
• Bacillus cereus trong sữa tiệt trùng
• Escherichia coli trong sữa tiệt trùng
• Biochemical assay
• GyrB gene
• CytK gene
• Bacillus cereus from milk
• Milk products
• Alkaline protease
• Bacillus cereus AG1
• Enzyme activity
• Detergent compatibility
• Soil isolate bacillus cereus AG1
• Maximum production of enzyme
• Submerged fermentation
• Optimization of amylase
• Production of amylase
• Bacillus cereus using submerged fermentation
• Antimicrobial activity
• Muthupet mangrove
• Novel Bacillus cereus isolated
• Mangrove ecosystem
• Enterotoxigenic bacillus cereus species isolated
• Conventional PCR
• Multiplex PCR
• Amplified PCR product
• Brown seaweeds
• Brown seaweed
• Sargassum wightii
• Isolation and characterization
• Exopolysaccharide producing bacillus cereus
• BMC Genomics
• Bacillus anthracis
• Bacillus cereus repeats
• Bacillus thuringiensis
• Group II introns
• Insertion sequences
• Antibiotic resistance
• B cereus
• Fresh vegetables
• Serious burden to public health
• Multidrug resistant Bacillus cereus
• Tiêu chuẩn ngành 10 TCN 731 2006
• Tiêu chuẩn ngành
• 10 TCN 731 2006
• Sn phẩm của thịt
• Xác định tổng số Bacillus cereus
• Bacillus cereus trong thịt
• Phospholipase C
• Pseudomonas aeruginosa
• Catalytic activity
• Cystic fibrosis
• Non random DNA Trimer arrangement
• Bacillus cereus chromosome ATCC 10987
• DNA arrangement
• Sense and antisense sequences
• Trimer reverse complement
• Chicken Briyani
• Exposure assessment
• Street food
• Deli Food of South India
• Luận văn Thạc sĩ
• Luận văn Thạc sĩ Sinh học
• Vi sinh vật học
• Ngộ độc thực phẩm
• Khuẩn lạc Bacillus cereus
• Cơ chế gây ngộ độc thực phẩm
• Thiol proteome
• Fermentative growth
• Differential labeling strategy
• Notorious foodborne pathogen
• vi khuẩn hiếu khí
• tổng nấm men
• Công nghiệp thực phẩm
• khuẩn lạc
• định lượng Bacillus cereus
• Wheat bran
• Agro industrial wastes
• Optimization of alkaline amylase production
• Scientific reports
• scientific research
• molecular Biology
• medicine
• cell proliferation
• oxidation
• Report medicine
• traditional medicine
• medical research
• hemoglobin
• medical knowledge
• phân tích vi sinh vật
• báo cáo khoa học
• báo cáo y học
• công trình nghiên cứu về y học
• tài liệu về y học
• cách trình bày báo cáo
• breast cancer
• biological activities
• environmental medicine