TAILIEUCHUNG - Genetic diversity and identification of some Turkish cotton genotypes (Gossypium hirsutum L.) by RAPD-PCR analysis

Cotton (Gossypium hirsutum L.) is the leading fiber crop and the second most important oil seed crop in the world. This study aimed to analyze the genetic differences among 9 cotton genotypes of cultivars from Turkey using the random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) method. | Y. SURGUN, B. ÇÖL, B. BÜRÜN Turk J Biol 36 (2012) 143-150 © TÜBİTAK doi: Genetic diversity and identification of some Turkish cotton genotypes (Gossypium hirsutum L.) by RAPD-PCR analysis Yonca SURGUN, Bekir ÇÖL, Betül BÜRÜN Department of Biology, Faculty of Science, Muğla University, 48000 Muğla - TURKEY Received: Abstract: Cotton (Gossypium hirsutum L.) is the leading fiber crop and the second most important oil seed crop in the world. This study aimed to analyze the genetic differences among 9 cotton genotypes of cultivars from Turkey using the random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) method. A total of 42 RAPD primers were used to determine polymorphism among the genotypes and 34 of the primers produced scorable results, with 319 RAPD-PCR bands obtained. The rate of polymorphism was calculated to be , and genetic similarity between any 2 genotypes ranged from to . By evaluating the presence and absence of the RAPD-PCR bands, a dendrogram indicating the relative genetic similarity of the Turkish cotton genotypes was constructed and presented. The results also showed that the genotypes can be separated from each other at the molecular level by taking advantage of some of the RAPD markers. Specific markers were proposed for the identification of genotypes. To our knowledge, this is the first study to use RAPD markers to investigate the genetic relationships among the Gossypium genotypes grown in Turkey. Key words: Cotton (Gossypium hirsutum L.), RAPD, genetic relationship Bazı Türk pamuk (Gossypium hirsutum L.) genotiplerinin RAPD-PZR analizi ile identifikasyonu ve genetik çeşitliliği Özet: Pamuk (Gossypium hirsutum L.) dünyada önde gelen lif bitkilerinden birisidir ve dünyada ikinci en önemli yağlı tohum kaynağıdır. Bu çalışmanın amacı, Türkiye’de kültürü yapılan 9 pamuk genotipinin genetik farklılığının RAPDPZR tekniği ile belirlenmesidir. Genotipler arasında, polimorfizmi saptamak

• Sinh học
• Cotton Gossypium hirsutum L
• Genetic relationship
• Random amplified polymorphic DNA
• Polymerase chain reaction method
• Gossypium genotypes
• Path coefficient Gossypium hirsutum
• Upland cotton (Gossypium hirsutum L )
• Upland cotton
• Path coefficient analysis
• Segregating population
• Path coefficient
• Seed cotton
• Cotton (Gossypium hirsutum L
• Seed cotton yield
• Yield attributing
• Fibre quality traits in cotton
• Genotype × environment
• Combining ability
• Gene action
• Seed cotton yield in cotton
• Environmental impact on the stability
• Gossypium hirsutum
• Basic lint yield traits
• Path analysis
• Heat stress regime
• Advance generation population
• Ginning out turn percent
• Lint yield kg/hectare
• Cotton (Gossypium hirsutum L )
• In vitro
• Osmotic treatment
• Cotton shoot regeneration
• Local cotton cultivar
• Stability analysis
• Component traits
• Additive effects
• Seed set
• Genetic variation among cotton
• Agricultural research institute
• Gossypium hirsutum (L )
• Line x tester
• Standard heterosis
• Component traits in upland cotton
• Plant growth regulators
• Production potential of cotton
• Production potential
• Maximizing inputs for cotton production
• Bt cotton
• Agronomic management practices
• Growth regulators
• Tield and economics
• Bt cotton (Gossypium hirsutum L
• Irrigated condition
• Character association
• Gossypium hirsutum L
• Quality traits in cotton
• Yield contributing
• Antioxidative enzyme
• Sucking pest
• Antioxidative responses in cotton
• Genotypes imparting resistance
• Sucking pest attack
• SSNM and RDF
• Productivity efficiency
• Bt cotton (Gossypium hirsutum L )
• SSNM based nutrition
• Date of sowing
• TBP irrigation command
• Lint quality as influenced
• Nutrient use efficiency
• Nutrient dynamics
• Leaf hopper
• Population dyanamics
• Gca and Sca effects
• Cotton (G hirsutum L )
• SCA effect and component traits
• Cotton (Gossypium hirsutumL )
• Component traits in cotton
• Indian economy
• Cultivars/genotypes
• Breeding programme
• American cotton (Gossypium hirsutum L )
• Genetic diversity in American cotton
• Biochemical parameters
• Seed germination
• Estimation of biochemical parameters
• Different environments
• Different environments in upland cotton
• Seed quality traits
• Environments in upland cotton
• Boll retention
• Sowing period
• Sowing conditions in upland cotton