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In vitro regeneration through seeds were studied in lime (Citrus aurantifolia) by culturing lime seeds on MS media supplemented with BAP (1, 3 and 5 mg/l), Kinetin (1, 3 and 5 mg/l) and their combination with NAA (0.1, 0.5 and 1 mg/l). In reference to increased germination percentage, mean shoot length, mean number of leaves per explant and mean root length, among BAP and Kinetin concentrations, BAP 3 mg/l was found to be the best treatment. In terms of cytokinins and NAA combine effect, BAP 5 mg/l + NAA 0.5 mg/l and Kinetin 3 mg/l + NAA at 0.1 mg/l were found to be the best combinations. Survival was 100 per cent when seeds were used as explant material. |