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Trong nghiên cứu này, các hoạt động tăng trưởng và trao đổi chất của Saccharomyces cerevisiae cố định trong gel alginate trong quá trình lên men rượu với ban đầu khác nhau nồng độ glucose đã được nghiên cứu. Bắt đầu từ nồng độ glucose ban đầu ở 140 g / L, chúng tôi quan sát thấy rằng việc tăng nồng độ này lên 220 g / L có ít ảnh hưởng đến sự phát triển của men cố định. | TAÏP CHÍ PHAÙT TRIEÅN KH&CN, TAÄP 11, SOÁ 12 - 2008 ALCOHOLIC FERMENTATION WITH DIFFERENT INITIAL GLUCOSE CONCENTRATION USING IMMOBILIZED YEAST IN CALCIUM ALGINATE GEL Bui Thanh Huyen, Le Van Viet Man University of Technology, VNU-HCM (Manuscript Received on March 27 th, 2006, Manuscript Revised Septemberl 03 rd, 2008) ABSTRACT: In this research, the growth and metabolic activities of Saccharomyces cerevisiae immobilized in alginate gel during alcoholic fermentation with different initial glucose concentration were studied. Starting from initial glucose concentration at 140 g/L, we observed that increasing this concentration to 220 g/L had little effect on the growth of immobilized yeast. Under the same conditions, the maximum biomass, glucose uptake rate, and final ethanol concentration obtained in the immobilized yeast cultures were always higher than those in the free yeast cultures. In 220 g glucose/L medium, the final ethanol concentration produced by immobilized yeast was very high (about 10.4-11.7% v/v). It can be concluded that immobilized yeast were less sensitive to high osmotic pressure and high ethanol concentration in medium than free yeast. Therefore, using immobilized yeast in alcoholic fermentation is potential to enhance ethanol productivity. 1.INTRODUCTION Ethanol has many uses in industry as well as in daily life. It is raw material in liquor production, a popular solvent in many industries and gradually becomes a popular environment friendly fuel. The development of the ethanol industry led to the demand for effective fermentation technique to improve ethanol yield. Nowadays, all Vietnamese ethanol factories carry out the alcoholic fermentation in medium with initial substrate concentration 150 g/L. High gravity fermentation technology allows production of higher ethanol concentration without changing plant size. However, in the culture with high initial substrate concentration, yeast growth and metabolic activities were significantly .
