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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Diagnostic value of anti-cyclic citrullinated peptides and association with HLA-DRB1 shared epitope alleles in African rheumatoid arthritis patients. | Singwe-Ngandeu et al. Arthritis Research Therapy 2010 12 R36 http arthritis-research.eom content 12 2 R36 RESEARCH ARTICLE Open Access Diagnostic value of anti-cyclic citrullinated peptides and association with HLA-DRB1 shared epitope alleles in African rheumatoid arthritis patients Madeleine Singwe-Ngandeu1 Axel Finckh2 Sylvette Bas2 4 Jean-Marie Tiercy3 4 Cem Gabay2 5 Abstract Introduction The purpose of this study was to examine the diagnostic performance of autoantibodies against citrullinated peptides proteins ACPA and to determine the prevalence of HLA-DRB1 shared epitope alleles SE in African patients with rheumatoid arthritis RA . Methods Serum levels of anti-cyclic citrullinated peptides antibodies anti-CCP2 anti-CCP3 IgM and IgA rheumatoid factors RF were measured by enzyme-linked immunosorbent assay in the serum of 56 consecutive RA patients regularly followed in the Rheumatology Unit of the School of Medicine University of Yaoundé Yaoundé Cameroon. Genotyping of HLA-DRB1 alleles was performed by polymerase chain reaction and hybridization with sequence-specific oligonucleotide probes on microbeads arrays. Fifty-one patients with other inflammatory rheumatic diseases and 50 healthy individuals were included as controls. Results An anti-CCP2 assay showed the best diagnosis sensitivity 82 and specificity 98 with high positive predictive PPV 96 and negative predictive values NPV 91 . Thirty percent of RA patients were carrying at least one copy of the HLA-DRB1 shared epitope SE compared to 10 and 14 of patients with other inflammatory rheumatic diseases and healthy individuals respectively. The presence of the SE was associated with the production of ACPA. Conclusions Anti-CCP2 antibodies are useful markers of RA in African patients. In this cohort the prevalence of the SE is higher in RA patients than in controls but lower than that reported in patient cohorts of European ancestry. The discrepancy between the high prevalence of ACPA-positive patients and .
