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Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Radiolabeling, biodistribution and gamma scintigraphy of noscapine hydrochloride in normal and polycystic ovary induced rats | Priyadarshani et al. Journal of Ovarian Research 2010 3 10 http www.ovarianresearch.eom content 3 1 10 RESEARCH JOURNAL OF OVARIAN RESEARCH Open Access Radiolabeling biodistribution and gamma scintigraphy of noscapine hydrochloride in normal and polycystic ovary induced rats Anjali Priyadarshani1 Krishna Chuttani2 Gaurav Mittal2 and Aseem Bhatnagar 2 Abstract Background Noscapine an alkaloid from Papaversomniferum widely used as an antitussive is being clinically studied in the treatment of polycystic ovary syndrome PCOS and a few other cancers primarily because of its antiangiogenesis properties. With the advent of diverse application of noscapine we sought to determine whether the radiolabeling method can be useful in studying uptake and kinetics of the molecule in-vivo. Specific objectives of this study were to radiolabel noscapine with Technetium-99m Tc-99m to determine its organ biodistribution in rat model and study its uptake kinetics in PCOS model. Methods A method for radiolabeling noscapine with Tc-99m was standardized using stannous reduction method and its in vitro and in vivo stability parameters were studied. The radiopharmaceutical was also evaluated for blood kinetics and biodistribution profile. An animal model of PCOS was created by using antiprogesterone RU486 and uptake of 99mTc-noscapine in normal and PCOS ovaries was compared using gamma scintigraphy. Results Noscapine hydrochloride was successfully radiolabeled with Tc-99m with high labeling efficiency and in vitro stability. Most of the blood clearance of the drug 80 took place in first hour after intravascular injection with maximum accumulation being observed in liver spleen kidney followed by the ovary. At 4 hours post injection radiolabeled complex accumulation doubled in PCOS ovaries in rats 0.9 0.03 ID whole organ compared to normal cyclic rats 0.53 0.01 ID whole organ . This observation was further strengthened by scintigraphic images of rats taken at different time intervals 1 h 2 h
