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Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Adeno-associated virus: from defective virus to effective vector | Virology Journal BioMed Central Review Open Access Adeno-associated virus from defective virus to effective vector Manuel AFV Gonẹalves Address Gene Therapy Section Department of Molecular Cell Biology Leiden University Medical Center Wassenaarseweg 72 2333 AL Leiden the Netherlands Email Manuel AFV Gonẹalves - m.goncalves@lumc.nl Corresponding author Published 06 May 2005 Received 08 April 2005 Accepted 06 May 2005 Virology Journal 2005 2 43 doi l0.ll86 l 743-422X-2-43 This article is available from http www.virologyj.eom content 2 1 43 2005 Gonẹalves licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The initial discovery of adeno-associated virus AAV mixed with adenovirus particles was not a fortuitous one but rather an expression of AAV biology. Indeed as it came to be known in addition to the unavoidable host cell AAV typically needs a so-called helper virus such as adenovirus to replicate. Since the AAV life cycle revolves around another unrelated virus it was dubbed a satellite virus. However the structural simplicity plus the defective and non-pathogenic character of this satellite virus caused recombinant forms to acquire centre-stage prominence in the current constellation of vectors for human gene therapy. In the present review issues related to the development of recombinant AAV rAAV vectors from the general principle to production methods tropism modifications and other emerging technologies are discussed. In addition the accumulating knowledge regarding the mechanisms of rAAV genome transduction and persistence is reviewed. The topics on rAAV vectorology are supplemented with information on the parental virus biology with an emphasis on aspects that directly impact on vector design and performance such .
