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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Epitope characterization of the protective monoclonal antibody VN04-2 shows broadly neutralizing activity against highly pathogenic H5N1 | Virology Journal BioMed Central Open Access Short report Epitope characterization of the protective monoclonal antibody VN04-2 shows broadly neutralizing activity against highly pathogenic H5N1 Angeline PC Lim Steven KK Wong Annie HY Chan Conrad EZ Chan Eng Eong Ooi and Brendon J Hanson Address Defence Medical and Environmental Research Institute DSO National Laboratories 27 Medical Dr. 117510 Singapore Email Angeline PC Lim - lpeichie@dso.org.sg Steven KK Wong - wkakhuen@dso.org.sg Annie HY Chan - choiyi@dso.org.sg Conrad EZ Chan - cenzuo@dso.org.sg Eng Eong Ooi - oengeong@dso.org.sg Brendon J Hanson - hbrendon@dso.org.sg Corresponding author Published II July 2008 Received 17 April 2008 Virology Journal 2008 5 80 doi 10.1186 1 743-422X-5-80 Accepted 11 July 2008 This article is available from http www.virologyj.cOm content 5 1 80 2008 Lim et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The monoclonal antibody VN04-2 was previously shown to protect mice against lethal A Vietnam 1203 04 H5N1 virus challenge when administered pre- and post-infection. In this study we characterized the binding requirements of this antibody using direct binding to hemagglutinin and neutralization assays with H5N1 virus-like particles H5N1-VLP of eight recent H5N1 strains representing the major mutations within the 140s antigenic loop. Binding was clade independent and 3 mutations within this antigenic region are required before escape is possible suggesting that apart from the H5N1 viruses circulating in Indonesia VN04-2 may provide protection against H5N1 viruses from all other regions. Findings In 1997 human disease was first reported due to .
