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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Ex vivo promoter analysis of antiviral heat shock cognate 70B gene in Anopheles gambiae | Virology Journal BioMed Central Open Access Ex vivo promoter analysis of antiviral heat shock cognate 70B gene in Anopheles gambiae Seokyoung Kang1 Cheolho Sim2 Brian D Byrd1 3 Frank H Collins4 and Young S Hong 1 Address Department of Tropical Medicine School of Public Health and Tropical Medicine Tulane University New Orleans Louisiana 70112 USA 2Department of Entomology the Ohio State University Columbus Ohio 43210 USA Environmental Health Sciences Western Carolina University Cullowhee NC 28723 USA and 4The Center for Global Health and Infectious Diseases University of Notre Dame Notre Dame IN 46556 USA Email Seokyoung Kang - skang1@tulane.edu Cheolho Sim - sim.15@osu.edu Brian D Byrd - bdbyrd@email.wcu.edu Frank H Collins - frank@nd.edu Young S Hong - young@tulane.edu Corresponding author Published 5 November 2008 Received 14 October 2008 Accepted 5 November 2008 Virologyjournal 2008 5 136 doi l0.ll86 l743-422X-5-l36 This article is available from http www.virologyj.com content 5 l l36 2008 Kang et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The Anopheles gambiae heat shock cognate gene hsc70B encodes a constitutively expressed protein in the hsp70 family and it functions as a molecular chaperone for protein folding. However the expression of hsc70B can be further induced by certain stimuli such as heat shock and infection. We previously demonstrated that the An. gambiae hsc70B is induced during o nyong-nyong virus ONNV infection and subsequently suppresses ONNV replication in the mosquito. To further characterize the inducibility of hsc70B by ONNV infection in An. gambiae we cloned a 2.6-kb region immediately 5 upstream of the starting codon of hsc70B into a luciferase reporter vector .
