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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Cutaneous and mucosal human papillomaviruses differ in net surface charge, potential impact on tropism | Virology Journal BioMed Central Open Access Short report Cutaneous and mucosal human papillomaviruses differ in net surface charge potential impact on tropism Nitesh Mistry Carl Wibom and Magnus Evander Address Department of Virology Umeâ University SE-901 85 Umeâ Sweden Email Nitesh Mistry-nitesh.mistry@ge.com Carl Wibom - carl.wibom@onkologi.umu.se Magnus Evander - magnus.evander@climi.umu.se Corresponding author Published 14 October 2008 Received 14 September 2008 Accepted 14 October 2008 Virology Journal 2008 5 118 doi l0.ll86 l743-422X-5-ll8 This article is available from http www.virologyj.com content 5 l l 18 2008 Mistry et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Papillomaviruses can roughly be divided into two tropism groups those infecting the skin including the genus beta PVs and those infecting the mucosa predominantly genus alpha PVs. The Ll capsid protein determines the phylogenetic separation between beta types and alpha types and the Ll protein is most probably responsible for the first interaction with the cell surface. Virus entry is a known determinant for tissue tropism and to study if interactions of the viral capsid with the cell surface could affect HPV tropism the net surface charge of the HPV Ll capsid proteins was analyzed and HPV-16 alpha and HPV-5 beta with a mucosal and cutaneous tropism respectively were used to study heparin inhibition of uptake. The negatively charged Ll proteins were all found among HPVs with cutaneous tropism from the beta- and gamma-PV genus while all alpha HPVs were positively charged at pH 7.4. The linear sequence of the HPV-5 Ll capsid protein had a predicted isoelectric point pI of 6.59 and a charge of -2.74 at pH 7.4 while HPV-16 had a pI of 7.95 .