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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Secretion of Epstein-Barr Virus-encoded BARF1 oncoprotein from latently infected B cells | Virology Journal BioMed Central Open Access Short report Secretion of Epstein-Barr Virus-encoded BARFI oncoprotein from latently infected B cells Sylvie Fiorini and Tadamasa Ooka Address Laboratoire de Virologie Moléculaire Virologie et Pathogenèse Humaine FRE 3011 CNRS Faculté de Médecine Laennec Université Claude Bernard Lyon-1 Rue Guillaume Paradin 69372 Lyon Cedex 08 France Email Sylvie Fiorini - sylvie.fiorini@sante.univ-lyon1.fr Tadamasa Ooka - ooka@sante.univ-lyon1.fr Corresponding author Published 4 June 2008 Received 18 February 2008 Virology Journal 2008 5 70 doi 10.1186 1743-422X-5-70 Accepted 4 June 2008 This article is available from http www.virologyj.cOm content 5 l 70 2008 Fiorini and Ooka licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Epstein-Barr virus EBV encodes two oncogenes LMP1 Latent Membrane Protein-1 and BARF1 BamH1-A Reading Frame-1 . LMP1 belongs to latent gene family and BARF1 is considered so far as one of early gene family. However BARF1 oncogene was expressed highly in Nasopharyngeal NPC and gastric GC carcinoma as a type II latency and in EBV-positive Akata cell and primary epithelial cell infected in vitro by EBV as type I latency. Its expression was also reported in Burkitt s lymphoma s biopsy frequent in Malawi in Africa as well as in nasal NK T-cell lymphoma. We recently observed a massive secretion of BARF1 protein in serum and saliva of NPC patients. NPC-derived c666-1 epithelial cells also expressed and secreted BARF1 protein without other lytic genes expression. We asked whether this oncogene belongs to latent gene family. To investigate we examined its transcriptional and translational expression in IB4 and Akata B cells where both cell lines belong to latent cell family.
