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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Alterations in intracellular potassium concentration by HIV-1 and SIV Nef | Virology Journal BioMed Central Open Access Research Alterations in intracellular potassium concentration by HIV-1 and SIV Nef Bongkun Choi 1 3 Cesar D Fermin2 Alla M Comardelle1 Allyson M Haislip1 Thomas G Voss1 and Robert F Garry1 Address Department of Microbiology and Immunology Tulane University Health Sciences Center New Orleans LA 70112 USA 2College of Veterinary Medicine Nursing Allied Health CVMNAH Tuskegee University Tuskegee AL 36088 USA and 3Departments of Environmental Medicine Pathology and Medicine New York University School of Medicine Tuxedo NY 10987 USA Email Bongkun Choi - choib01@med.nyu.edu Cesar D Fermin - fermin_c@tuskegee.edu Alla M Comardelle - a_comardelle@yahoo.com Allyson M Haislip - amh77@tulane.edu Thomas G Voss - tvoss@tulane.edu Robert F Garry - rfgarry@tulane.edu Corresponding author Published 19 May 2008 Received 5 May 2008 Virology Journal 2008 5 60 doi 10.1186 1743-422X-5-60 Accepted 19 May 2008 This article is available from http www.virologyj.cOm content 5 1 60 2008 Choi et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background HIV-1 mediated perturbation of the plasma membrane can produce an alteration in the transmembrane gradients of cations and other small molecules leading to cell death. Several HIV-1 proteins have been shown to perturb membrane permeability and ion transport. Xenopus laevis oocytes have few functional endogenous ion channels and have proven useful as a system to examine direct effects of exogenously added proteins on ion transport. Results HIV-1 Nef induces alterations in the intracellular potassium concentration in CD4 T-lymphoblastoid cells but not intracellular pH. Two electrode voltage-clamp recording was used to determine that Nef did not form
