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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Varicella-zoster virus ORF 58 gene is dispensable for viral replication in cell culture | Virology Journal BioMed Central Research Open Access Varicella-zoster virus ORF 58 gene is dispensable for viral replication in cell culture Hironori Yoshii1 2 Kay Sadaoka1 Masaaki Matsuura1 2 Kazuhiro Nagaike2 Michiaki Takahashi3 Koichi Yamanishi1 and Yasuko Mori 1 Address laboratory of Virology and Vaccinology Division of Biomedical Research National Institute of Biomedical Innovation Osaka Japan 2Kanonji Institute the Research Foundation for Microbial Diseases of Osaka University Kanonji Kagawa Japan and 3The Research Foundation for Microbial Diseases of Osaka University Suita Osaka Japan Email Hironori Yoshii - hyoshii@mail.biken.or.jp Kay Sadaoka - kaysada@nibio.go.jp Masaaki Matsuura - mmatsuura@nibio.go.jp Kazuhiro Nagaike - knagaike@mail.biken.or.jp Michiaki Takahashi - mtakahashi@mail.biken.or.jp Koichi Yamanishi - yamanishi@nibio.go.jp Yasuko Mori - ymori@nibio.go.jp Corresponding author Published 30 April 2008 Received 4 January 2008 Accepted 30 April 2008 Virology Journal 2008 5 54 doi l0.ll86 l 743-422X-5-54 This article is available from http www.virologyj.cOm content 5 1 54 2008 Yoshii et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Open reading frame 58 ORF58 of varicella-zoster virus VZV lies at the 3 end of the Unique long UL region and its functional is unknown. In order to clarify whether ORF58 is essential for the growth of VZV we constructed a deletion mutant of ORF58 pOka-BACA58 from the Oka parental genome cloned into a bacterial artificial chromosome pOka-BAC . Results The ORF58-deleted virus rpOkaA58 was reconstituted from the pOka-BACA58 genome in MRC-5 cells indicating that the ORF58 gene is non-essential for virus growth. Comparison of the growth rate of rpOkaA58 and .
