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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Apolipoprotein E-specific innate immune response in astrocytes from targeted replacement mice | Journal of Neuroinflammation BioMed Central Research Open Access Apolipoprotein E-specific innate immune response in astrocytes from targeted replacement mice Izumi Maezawa1 Nobuyo Maeda2 Thomas J Montine1 and Kathleen S Montine 1 Address Department of Pathology University of Washington Seattle WA USA and 2Department of Pathology University of North Carolina Chapel Hill NC USA Email Izumi Maezawa - imaezawa@u.washington.edu Nobuyo Maeda - nobuyo@med.unc.edu Thomas J Montine - tmontine@u.washington.edu Kathleen S Montine - kmontine@u.washington.edu Corresponding author Published 07 April 2006 Received 08 March 2006 Journal of Neuroinflammation 2006 3 10 doi l0.ll86 1742-2094-3-10 Accepted 07 ApnI 2006 This article is available from http www.jneuroinflammation.cOm content 3 1 10 2006 Maezawa et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Inheritance of the three different alleles of the human apolipoprotein apo E gene APOE are associated with varying risk or clinical outcome from a variety of neurologic diseases. ApoE isoform-specific modulation of several pathogenic processes in addition to amyloid p metabolism in Alzheimer s disease have been proposed one of these is innate immune response by glia. Previously we have shown that primary microglia cultures from targeted replacement TR APOE mice have apoE isoform-dependent innate immune activation and paracrine damage to neurons that is greatest with TR by the e4 allele TR APOE4 and that derives from p38 mitogen-activated protein kinase p38MAPK activity. Methods Primary cultures of TR APOE2 TR APOE3 and TR APOE4 astrocytes were stimulated with lipopolysaccharide LPS . ApoE secretion cytokine production and nuclear factor-kappa B NF-kB subunit .
