Đang chuẩn bị nút TẢI XUỐNG, xin hãy chờ
Tải xuống
Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Conformational epitopes of myelin oligodendrocyte glycoprotein are targets of potentially pathogenic antibody responses in multiple sclerosis | Menge et al. Journal of Neuroinflammation 2011 8 161 http www.jneuroinflammation.eom content 8 1 161 JOURNAL OF NEUROINFLAMMATION RESEARCH Open Access Conformational epitopes of myelin oligodendrocyte glycoprotein are targets of potentially pathogenic antibody responses in multiple sclerosis Til Menge1 2 Patrice H Lalive1 3 H-Christian von Budingen 1 and Claude P Genain 1 Abstract Background Myelin oligodendrocyte glycoprotein MOG is a putative autoantigen in multiple sclerosis MS . Establishing the pathological relevance and validity of anti-MOG antibodies as biomarkers has yielded conflicting reports mainly due to different MOG isoforms used in different studies. Because epitope specificity may be a key factor determining anti-MOG reactivity we aimed at identifying a priori immunodominant MOG epitopes by monoclonal antibodies mAbs and at assessing clinical relevance of these epitopes in MS. Methods Sera of 325 MS patients 69 patients with clinically isolated syndrome and 164 healthy controls were assayed by quantitative high-throughput ELISA for reactivity to 3 different MOG isoforms and quantitative titers correlated with clinical characteristics. mAbs defined unique immunodominant epitopes distinct to each of the isoforms. Results In the majority of human samples anti-MOG levels were skewed towards low titers. However in 8.2 of samples high-titer anti-MOG antibodies were identified. In contrast to anti-MOG reactivity observed in a mouse model of MS in patients with MS these never reacted with ubiquitously exposed epitopes. Moreover in patients with relapsing-remitting MS high-titer anti-MOG IgG correlated with disability EDSS Spearman r 0.574 p 0.025 . Conclusions Thus high-titer reactivity likely represents high-affinity antibodies against pathologically relevant MOG epitopes that are only present in a small proportion of patients with MS. Our study provides valuable information about requirements of anti-MOG reactivity for being regarded as a prognostic .
